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A kit and method for detecting yak-derived ingredients

A technology of source components and kits, applied in the field of molecular biology, can solve the problems of inability to make accurate determinations, fuzzy determination of test results, and insufficient specificity of primer sequences.

Inactive Publication Date: 2019-01-25
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The existing standard "SNT 4397-2015 Real-time Fluorescent PCR Method for Detection of Yak-derived Components in Exported Foods" uses the fluorescent PCR method to detect yak-derived components, but it can only be used for export foods, and we found during the testing process that This method will have false positives for pork components, and the accuracy of the determination results cannot be guaranteed
[0005] The patent application with the publication number CN 102634581 A discloses a fluorescent quantitative PCR method for yak-derived fiber components. It only performs PCR amplification for yak hair, and the primer sequence is not specific enough, which leads to ambiguity in the determination of the detection results and cannot be accurately determined. determination

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  • A kit and method for detecting yak-derived ingredients
  • A kit and method for detecting yak-derived ingredients
  • A kit and method for detecting yak-derived ingredients

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Embodiment 1

[0050] Example 1 Kit and detection method for detecting yak-derived components of the present invention

[0051] One, kit composition of the present invention

[0052] Including the primer pair shown in SEQ ID NO.1, SEQ ID NO.2 and the probe shown in SEQ ID NO.3; as shown in Table 1:

[0053] Table 1 Primers and probes of the present invention

[0054]

[0055] Note: F is the 5' end primer, R is the 3' end primer, and P is the probe.

[0056] Two, adopt the kit of the present invention to detect yak-derived components

[0057] 1. DNA extraction

[0058] After the sample is homogenized, weigh 0.1g to 0.2g, put it in a 2mL centrifuge tube, add 600μL CTAB lysate and 20μL proteinase K, shake and mix, incubate at 65°C for 1h to 2h, and shake and mix every 10min during the period; add 500 μL of phenol: chloroform: isoamyl alcohol (25:24:1), fully shake and mix, centrifuge at 12000g for 5 minutes; carefully pipette the supernatant into a clean 1.5mL centrifuge tube, add an equ...

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Abstract

The present invention relates to a kit for detecting yak-derived components comprising a primer pair shown in SEQ ID NO. 1 to SEQ ID NO. 2 and a probe shown in SEQ ID NO. 3, and also to a method for detecting yak-derived components. The kit and the method of the invention can accurately detect yak-derived ingredients in meat and meat products, and the kit and the method have the advantages of fastdetection, accuracy, low cost, good specificity, high sensitivity, and wide application prospect.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a kit and method for detecting yak-derived components. Background technique [0002] In recent years, with the rapid expansion of the meat market, incidents of meat adulteration have occurred frequently. There are more and more ways of adulteration, and the forms are becoming more and more complicated. It has become a prominent problem in the global food industry. The adulteration of meat products will not only cause individual health risks, including disease infection, metabolic disorders, allergic reactions, etc., but also more likely to cause economic, religious, and moral problems. [0003] At present, common detection techniques for meat adulteration include ELISA, microscopic determination, electronic nose, mass spectrometry, etc., but they are not widely used because of their shortcomings such as low sensitivity, long cycle time, and inability to detect cooked ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2563/107
Inventor 唐卓段庆梓杜凤梁恒兴陈浩东黄春燕陈蓉张彪董娟张玉尚柯王巍吴文林
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S