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A kit and method for detecting goat-derived ingredients

A technology of source components and kits, applied in the field of molecular biology, can solve the problems of inability to determine the logarithmic growth period, poor amplification curve, and inability to guarantee the accuracy of the results, and achieve low cost, rapid detection, and high sensitivity. Effect

Inactive Publication Date: 2019-01-25
成都市食品药品检验研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chen Yongfeng (Chen Yongfeng, the establishment of a dual fluorescent PCR detection method for goat-derived and sheep-derived components in feed and animal foods [J], Fujian Animal Husbandry and Veterinary Medicine, 2012, 3:1-4) reported a method of dual fluorescent PCR The method for detecting goat-derived components has a poor amplification curve in the result, the logarithmic growth period cannot be determined, and the accuracy of the result cannot be guaranteed

Method used

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  • A kit and method for detecting goat-derived ingredients
  • A kit and method for detecting goat-derived ingredients
  • A kit and method for detecting goat-derived ingredients

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Embodiment 1

[0051] Example 1 Kit and detection method for detecting goat-derived components of the present invention

[0052] One, kit composition of the present invention

[0053] Including the primer pair shown in SEQ ID NO.1, SEQ ID NO.2 and the probe shown in SEQ ID NO.3; as shown in Table 1:

[0054] Table 1 Primers and probes of the present invention

[0055]

[0056] Note: F is the 5' end primer, R is the 3' end primer, and P is the probe.

[0057] Two, using the kit of the present invention to detect goat-derived components

[0058] 1. DNA extraction

[0059] After the sample is homogenized, weigh 0.1g to 0.2g, put it in a 2mL centrifuge tube, add 600μL CTAB lysate and 20μL proteinase K, shake and mix, incubate at 65°C for 1h to 2h, and shake and mix every 10min during the period; add 500 μL of phenol: chloroform: isoamyl alcohol (25:24:1), shake well, centrifuge at 12 000 g for 5 min; carefully pipette the supernatant into a clean 1.5 mL centrifuge tube, add an equal volum...

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Abstract

The invention relates to a kit for detecting goat-derived ingredients comprising primer pairs shown in SEQ ID NO. 1 to SEQ ID NO. 2 and probes shown in SEQ ID NO. 3, and a method for detecting goat-derived ingredients. The kit and the method of the invention can accurately detect goat-derived ingredients in meat and meat products, and the kit and the method have the advantages of rapid detection,simplicity, low cost, high specificity and sensitivity, and good application prospect.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a kit and method for detecting goat-derived components. Background technique [0002] In recent years, with the rapid expansion of the meat market, incidents of meat adulteration have occurred frequently. There are more and more ways of adulteration, and the forms are becoming more and more complicated. It has become a prominent problem in the global food industry. Adulteration of meat products will not only cause individual health risks, including disease infection, metabolic disorders, allergic reactions, etc., but also more likely to cause economic, religious, and moral problems. [0003] At present, common detection techniques for meat adulteration include ELISA, microscopic determination, electronic nose, mass spectrometry, etc., but they are not widely used because of their shortcomings such as low sensitivity, long cycle time, and inability to detect cooked meat. PCR tech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888
Inventor 王巍张敏唐卓梁恒兴杜凤段庆梓陈浩东吴文林黄春燕张彪张玉尚柯
Owner 成都市食品药品检验研究院