Method for evaluating endocrine disruption effects in water environment based on zebra fish hormone level response
An endocrine disruption and evaluation method technology, applied in the field of quantitative assessment of endocrine disruption effects in water environment, can solve problems such as inability to evaluate compound effects, inability to comprehensively reflect water environment health risks, and inability to identify pollutants in water environment health risk assessment models.
Inactive Publication Date: 2019-01-29
ZHEJIANG UNIV
View PDF7 Cites 2 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, in practical applications, this type of health risk assessment method based on pollutant concentration indicators has certain shortcomings: first, it is often impossible to identify all pollutants in the water environment in practical applications; second, not all pollutants have Corresponding toxicity test data; 3. Unable to evaluate compound effects
Due to these limitations, such water environment health risk assessment models often cannot comprehensively reflect the real water environment health risks under actual conditions.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0011] Zebrafish sterol hormones, amino acid derivative hormones, fatty acid derivative hormones and other hormones are used as biomarkers for the evaluation of endocrine disrupting effects in water environments, and their differential expression can be determined using ultra-high performance liquid chromatography-electrospray ionization-triple The quadrupole tandem mass spectrometer realizes the quantitative analysis, and then conducts unified quantification through the zebrafish hormone response index to intuitively and comprehensively evaluate the grade and degree of the endocrine disrupting effect of the water environment. The method does not need to determine the type and concentration of pollutants, has the advantages of saving time, labor and money, and can achieve high sensitivity, high accuracy and high throughput.
[0012] 1. Selection of Zebrafish Hormone Biomarkers
[0013] A variety of hormone biomarkers were selected from three aspects: steroid hormones, amino ac...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a method for evaluating endocrine disruption effects in water environment based on zebra fish hormone level response. The invention firstly performs zebra fish exposure experiment, then simultaneously quantitatively analyzes various protein biomarkers in zebra fish by liquid chromatography-mass spectrometry, and quantifies the biomarker response by zebra fish protein response index to determine the health risk rating and level of the exposed water body. The method provided by the invention measures the differential expression of various hormones in zebra fish exposed towater environment medium, and quantifies each hormone biomarker into a zebra fish hormone response index to quantitatively characterize the endocrine disrupting effect of the water environment.
Description
technical field [0001] The invention belongs to the technical field of environmental monitoring and risk assessment, and relates to a water environment endocrine interference effect evaluation method using hormone levels as indicators, in particular to a comprehensive quantification of various hormone-like organisms in zebrafish (Daniorerio) exposed to water environment media Differential expression of markers, and a method for quantitatively assessing the endocrine disrupting effect of water environment. Background technique [0002] The water environment is an important part of human survival and development. However, since 1993, about 53% of the more than 1,000 serious and serious environmental emergencies that have occurred in my country are water pollution accidents. Compared with the increasingly serious water environment pollution risk, my country's water environment risk assessment ability is relatively weak. Health risk assessment originated in the 1980s. It uses r...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N33/186
Inventor 杨方星
Owner ZHEJIANG UNIV