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Extracting method for nux prinsepiae fruit polysaccharides

An extraction method and fruit technology, which are applied in the field of polysaccharide extraction from Rutabaga fruit, can solve the problems of rare polysaccharides, and achieve the effects of small loss of polysaccharides, high yield, and simple method

Inactive Publication Date: 2019-02-05
晋燕君
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, the researches on Rutacea at home and abroad are mainly in the observation of biological characteristics, cultivation technology, greening and environmental protection, edible and medicinal, etc. The research on the components of Rutabaga is limited to the study of its alcohol-soluble components, but the polysaccharide research is rare

Method used

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  • Extracting method for nux prinsepiae fruit polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for extracting polysaccharides from rutabagas, comprising the steps of:

[0029] (1) Pretreatment: wash the rutabaga fruit, dry it in an oven at 60°C, grind it into powder in a mortar, and pass it through a 60-mesh sieve;

[0030] (2) Ultrasonic extraction: Weigh 5g of fruit powder into a beaker, add 150mL of water, shake well, conduct ultrasonic extraction in a 30°C water bath for 45min, filter, add 150mL of water to the filter residue, repeat ultrasonic extraction twice, and combine three times of extraction liquid;

[0031] (3) Centrifugal filtration: place the extract in a centrifuge at a speed of 4500r / min, centrifuge for 15min, then filter, and take the supernatant;

[0032] (4) Concentration and decolorization: heat and concentrate the supernatant until the volume of the filtrate is reduced to 30mL, then transfer the concentrated solution into a large test tube, add 3 times the volume of hydrogen peroxide to heat and decolorize, so that the light yellow...

Embodiment 2

[0038] As shown in Example 1, other conditions remain unchanged, and the extraction time is 35 minutes. After using this method for extraction, the extraction rate of rutabaga oil is 84%.

Embodiment 3

[0040] As shown in Example 2, other conditions remain unchanged, and the extraction temperature is 40°C. After extraction by this method, the extraction rate of rutabaga oil is 83%.

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Abstract

The invention relates to the technical field of polysaccharide extraction, and discloses an extracting method for nux prinsepiae fruit polysaccharides. The method comprises the following steps: pre-treating nux prinsepiae fruits, performing ultrasonic extraction, performing centrifugal filtration, concentrating and discolouring, removing proteins, purifying, and performing centrifugal drying. Themethod has the beneficial effects that the nux prinsepiae fruit drying temperature is 60 DEG C, so that the polysaccharide component is ensured not to be damaged; interference of an outside environment is reduced after the nux prinsepiae fruits are treated, sealed and stored; ultrasonic extraction time is short, the yield is high, and heating is not added; hydrogen peroxide is utilized for discolouring, the method is simple, operations are convenient, and loss of polysaccharides is relatively small; and chloroform-n-butyl alcohol process is used to remove proteins, so that degradation of polysaccharides can be avoided, and therefore, polysaccharides can be stabilized.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to a method for extracting polysaccharides from rutabaga fruit. Background technique [0002] Rutabaga is the dry and mature fruit stone of Rosaceae plants or Cystia serrata. It is mainly produced in Shanxi, Shaanxi, Gansu, Inner Mongolia and other places. It is a valuable medicinal plant with broad development prospects. [0003] Ruihe is a traditional Chinese medicine. It is sweet in taste, slightly cold in nature, and returns to the heart and liver meridian. disturbed. The ancients mainly used it for the treatment of eye diseases. Modern clinical application has proved that the traditional Chinese medicine with rutabagas as an auxiliary material has a certain effect on the treatment of cataracts, and has a good clinical effect on the treatment of vertigo due to hyperactivity of liver yang. However, at present, the researches on Rutacea at home and abroad are ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 晋燕君
Owner 晋燕君