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Device and method for measuring defocus distance of micro-scale transparent body based on additional phase plate

A technology of measuring device and phase plate, which is applied to measuring device, phase influence characteristic measurement, using optical device, etc. degree and other issues to achieve the effect of improving stability

Active Publication Date: 2020-11-03
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing parameters for measuring defocus degree have complex relationships with imaging system parameters, imaging background and imaging objects, and it is difficult to establish a definite relationship between defocus degree parameters and defocus distance, so it is difficult to measure by the absolute quantity of these parameters To determine the degree of defocus of the imaging object, it is necessary to first establish the relationship curve between the defocus degree parameter and the defocus distance, and then compare the measurement and calculation results with the relationship curve to determine the defocus degree of the imaging object (in the direction of the optical axis relative to position of the objective lens)

Method used

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  • Device and method for measuring defocus distance of micro-scale transparent body based on additional phase plate
  • Device and method for measuring defocus distance of micro-scale transparent body based on additional phase plate
  • Device and method for measuring defocus distance of micro-scale transparent body based on additional phase plate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 (|( SPEC 2 -SPEC 1 ) / (z 2 -z 1 )|The largest case, with Figure 5 )

[0047] (t 2 -t 1 )×(n-n 0 ), (t 3 -t 2 )×(n-n 0 ) and (t 4 -t 3 )×(n-n 0 ) take values ​​of 145 μm, 147 μm, and 150 μm, respectively. Calculated parameters obtained from the phase diagram: SPEC 1 =1810,z 1 = 0; SPEC 2 =1580,z 2 =145μm; SPEC 3 =1795,z 3 =292μm; SPEC 4 =1985,z 4 = 442 μm. The calculated absolute value of the slope of the straight line formed by adjacent measurement points: |(SPEC 2 -SPEC 1 ) / (z 2 -z 1 )|=1.586, |(SPEC 3 -SPEC 2 ) / (z 3 -z 2 )|=1.463 and |(SPEC 4 -SPEC 3 ) / (z 4 -z 3 )|=1.267. Judging|(SPEC 2 -SPEC 1 ) / (z 2 -z 1)|Maximum, the formula used to determine the relative position of the image plane of the imaging system is According to the formula, z=150.73 μm is calculated. Then according to the formula 1 / (1 / f-1 / v)-t 1 ×(n-n 0 )+z calculates that the distance between the phase body and the objective lens is 9.65073mm.

Embodiment 2

[0048] Example 2 (|(SPEC 4 -SPEC 3 ) / (z 4 -z 3 )|The largest case, with Figure 6 )

[0049] (t 2 -t 1 )×(n-n 0 ), (t 3 -t 2 )×(n-n 0 ) and (t 4 -t 3 )×(n-n 0 ) take values ​​of 157 μm, 144 μm, and 150 μm, respectively. Calculated parameters obtained from the phase diagram: SPEC 1 =1960,z 1 = 0; SPEC 2 =1770,z 2 =157μm; SPEC 3 =1500,z 3 =301μm; SPEC 4 =1860,z 4 = 451 μm. The calculated absolute value of the slope of the straight line formed by adjacent measurement points: |(SPEC 2 -SPEC 1 ) / (z 2 -z 1 )|=1.210,|(SPEC 3 -SPEC 2 ) / (z 3 -z 2 )|=1.875 and |(SPEC 4 -SPEC 3 ) / (z 4 -z 3 )|=2.4. Judging|(SPEC 4 -SPEC 3 ) / (z 4 -z 3 )|Maximum, the formula used to determine the relative position of the image plane of the imaging system is According to the formula, z=285.25 μm is calculated. Then according to the formula 1 / (1 / f-1 / v)-t 1 ×(n-n 0 )+z calculates that the distance between the phase body and the objective lens is 9.78525mm.

Embodiment 3

[0050] Example 3 (|(SPEC 3 -SPEC 2 ) / (z 3 -z 2 )|Maximum and SPEC 4 >SPEC 1 case, with Figure 7 )

[0051] (t 2 -t 1 )×(n-n 0 ), (t 3 -t 2 )×(n-n 0 ) and (t 4 -t 3 )×(n-n 0 ) take values ​​of 144 μm, 150 μm, and 156 μm, respectively. Calculated parameters obtained from the phase diagram: SPEC 1 =1770,z 1 = 0; SPEC 2 =1500,z 2 =144μm; SPEC 3 =1860,z 3 =294μm; SPEC 4 =2010,z 4 = 450 μm. The calculated absolute value of the slope of the straight line formed by adjacent measurement points: |(SPEC 2 -SPEC 1 ) / (z 2 -z 1 )|=1.875, |(SPEC 3 -SPEC 2 ) / (z 3 -z 2 )|=2.4 and |(SPEC 4 -SPEC 3 ) / (z 4 -z 3 )|=0.962. Judging|(SPEC 3 -SPEC 2 ) / (z 3 -z 2 )|Maximum and SPEC 4 >SPEC 1 , the formula used to determine the relative position of the image plane of the imaging system is According to the formula, z=128.25 μm is calculated. Then according to the formula 1 / (1 / f-1 / v)-t 1 ×(n-n 0 )+z calculates that the distance between the phase body and the ...

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Abstract

The invention discloses a micro-scale transparent body defocusing distance measuring device and method based on an additional phase sheet, and relates to the technical field of micro-scale transparentbody testing, specifically, through arrangement of additional phase sheets, phase body images at four different defocusing degrees are obtained, SPEC parameters are calculated based on the images todetermine the position of the phase body relative to an imaging system objective lens in the optical axis direction. Real-time measuring of the transparent phase body three-dimensional spatial position is achieved. The rotating phase sheet is added to a light path, and therefore light path adjusting can be achieved without the mechanical movement of the light path, and stability of the system is improved. The direct calculating mode is adopted, the iterative optimization process is not required, and the calculating process is simple.

Description

technical field [0001] The invention relates to the technical field of micro-scale transparent body testing, in particular to a system and method for measuring the position of a transparent body relative to a lens through four measurements after adding a phase plate, which is applicable to the measured micro-scale transparent body relative to the lens. The background medium is the measurement of the position of the transparent body with different refractive indices in the direction of the optical axis. Background technique [0002] The three-dimensional position information of an object is the basic data to determine the law of the object's movement, and plays an important role in the fields of biology, physics, chemistry, mechanical engineering and information. Among them, the three-dimensional position of the micro-scale transparent body (represented by cells) The measurement of is the basis of biological research. [0003] At present, in order to realize the measurement ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01B11/00G01N21/45
CPCG01B11/00G01N21/45
Inventor 金卫凤李健王亚伟
Owner JIANGSU UNIV