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A fermented carbonized honeysuckle granule and its preparation method and application

A honeysuckle and carbonization technology, which is applied in the field of fermented and carbonized honeysuckle granules and its preparation, can solve problems such as inability to add flavor to cigarettes, ineffective adsorption of harmful substances in smoke, and poor coordination of cigarette aromas, etc. Dual effects of fragrance and coke reduction, good commercial application value

Active Publication Date: 2021-01-29
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the disadvantages of the existing plant particles, such as insignificant adsorption effect on smoke harmful substances, inability to directly add aroma to cigarettes, or poor coordination with cigarette aroma, etc., the present invention provides a fermented carbonized honeysuckle granule and its preparation method and application. By modifying the plant raw materials by using the fermenting and carbonization process of the tobacco Soil Dainella L4-6, it is possible to provide a product that can not only enhance the effect of cigarette harm reduction and tar reduction, reduce the irritation of cigarette smoke, but also enhance the effect of cigarette flavoring and enhance the quality of cigarettes. cigarette smoking quality

Method used

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  • A fermented carbonized honeysuckle granule and its preparation method and application
  • A fermented carbonized honeysuckle granule and its preparation method and application
  • A fermented carbonized honeysuckle granule and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]1. Isolation, cultivation and identification of Tobacco Solanum Daiia L4-6

[0040] 1.1 Isolation of Tobacco Soil Daeella L4-6

[0041] Tobacco soil samples were taken from the tobacco planting base in Kunming, Yunnan, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 10g of soil sample, add 90mL of sterile water, shake on a shaker at 30°C and 180rpm for 30min; dilute the concentration to 10 with sterile water -1 、10 -2 、10 -3 、10 -4 、10 -5 Take 0.2mL of the five concentrations of bacterial solution and spread it on the LB plate medium, set up three parallel experiments for each concentration of the bacterial solution, cultivate it at 30°C for 48 hours, and pick it from the plate of each soil sample For different single colonies, after purification on LB plate medium, 20% of the bacterial volume was added to glycerol, and stored in a -80°C refrigerator for later use.

[0042] The isolated microorganisms were respectively inoculated in LB liq...

Embodiment 2

[0058] 1. Cultivation of Tobacco Soil D. L4-6

[0059] (1) Test tube slant culture

[0060] Slope preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: 0.5g of glucose, 0.5g of yeast extract, 0.5g of peptone, 0.5g of acid hydrolyzed casein, 0.5g of soluble starch, and 0.3g of sodium pyruvate , dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on a slant, inoculate it with the strain L4-6 of Agrobacterium nicotianae, and cultivate it at 28°C for 1 week to obtain the strains in the test tube;

[0061] (2) Seed cultivation

[0062] Adopt seed medium, the formula of seed medium is: dextrin 120g, soybean powder 40g, yeast extract 2g, tryptophan 0.5g, β-alanine 5g, magnesium sulfate 0.5g, ammonium phosphate 0.2g, distilled water constant volume to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, pick pa...

Embodiment 3

[0073] 1. The culture method of Tobacco Soil Dainella L4-6 is the same as in Example 2.

[0074] 2. Fermentation of honeysuckle

[0075] Weigh 600g of honeysuckle with pre-balanced water content to 12%, spray 120mL of inoculum of Agrobacterium nicota L4-6, put the treated honeysuckle in a 22°C, 60% constant temperature and humidity box to ferment for 72h.

[0076] 3. Carbonization of fermented honeysuckle

[0077] Put the fermented honeysuckle into the carbonization furnace, gradually raise the temperature of the furnace to 80°C, and keep it for 30 minutes. After the honeysuckle fermentation sample is dried, quickly raise the temperature to 130°C, and keep it for 3 hours. After checking the degree of carbonization, turn off the heat source, and take out the fermentation carbonization The honeysuckle samples are ready for use.

[0078] 4. Preparation of fermented carbonized honeysuckle granules

[0079] Take fermented carbonized honeysuckle, detect its water content, make up...

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Abstract

The invention provides a fermented carbonized honeysuckle granule and its preparation method and application, which are prepared by the method comprising the following steps: (1) preparation of tobacco soil Daisybacteria L4‑6 inoculum; (2) using tobacco soil Daisynia Bacteria L4-6 bacteria agent ferments honeysuckle to obtain fermented honeysuckle; (3) carbonizes fermented honeysuckle to obtain fermented carbonized honeysuckle; (4) places fermented carbonized honeysuckle powder on a fluidized bed to dry, and sprays adhesive , bond powder to form granules, and continue drying to obtain fermented carbonized honeysuckle granules. The granules are fermented and applied to cigarettes to produce a special aroma that harmonizes with cigarette smoke. While enriching the aroma of cigarettes, it also has pharmacological effects such as relieving sore throat, clearing heat and reducing inflammation; after carbonization treatment, it can significantly increase the aroma of honeysuckle The internal adsorption specific surface area strengthens the adsorption of particles to harmful substances in cigarette smoke, and at the same time endows plant particles with a special burnt aroma.

Description

technical field [0001] The invention belongs to the technical field of cigarette materials, and in particular relates to fermented carbonized honeysuckle granules and a preparation method and application thereof. Background technique [0002] Low coking has become an inevitable trend in the development of the tobacco industry. Low coking brings about a reduction in the taste of cigarettes, and insufficient aroma has gradually become a prominent problem in the development of tobacco. How to overcome the key technologies that affect the aroma and aroma of tobacco leaves has become the core of improving the competitiveness of cigarette production. Adding tobacco flavors and fragrances to shredded cigarettes is a conventional and necessary technical means to add aroma, but the space for improving the quality of cigarettes is relatively limited. It has become a new development trend to improve the aroma of cigarettes and reduce the irritation of cigarette smoke. Among them, add...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24D3/14C01B32/05C12N1/20C12R1/01
CPCA24D3/14C12N1/20C01B32/05
Inventor 高莉段焰青夏建军李文均焦俊杜刚李先毅
Owner CHINA TOBACCO YUNNAN IND
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