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Drug composition for treating neuroblastoma and neuroglioma and application thereof

The technology of a composition and drug, applied in the field of tumor treatment, can solve the problems such as no report of combined drug use of statin and abiraterone acetate

Active Publication Date: 2019-03-01
王阳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In vitro studies have confirmed that the two drugs have a certain inhibitory effect on the proliferation of glioma when used alone, but there is no report on the combination of statin and abiraterone acetate, nor the use of them to inhibit the proliferation of neuroblastoma

Method used

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  • Drug composition for treating neuroblastoma and neuroglioma and application thereof
  • Drug composition for treating neuroblastoma and neuroglioma and application thereof
  • Drug composition for treating neuroblastoma and neuroglioma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: specific experimental scheme

[0028]1) Cell culture: Human NB cell line SH-SY5Y, mouse NB cell line Neuro2A, human glioma cell lines T98G, U87MG, U118MG were cultured in DMEM, while human NB cell line SK-N-BE(2) was cultured in In DMEM / F12 (50 / 50), 10% FBS and 1% penicillin-streptomycin were added to DMEM or DMEM / F12, and the cells were cultured in a 96-well plate (100 μl medium per well), 5% CO 2 , at 37°C.

[0029] 2) Drug treatment: statin was dissolved in DMSO, abiraterone acetate was dissolved in ethanol (the drug was purchased from MCE, China), and the cell viability was detected after adding the drug at a specified concentration to the culture system for 72 hours. Each concentration of each drug was set at 5 parallel holes.

[0030] 3) Cell viability detection: MTT method is used to detect the cell viability of drugs alone or in combination. After 72 hours of drug treatment, add 20ul of MTT solution (5mg / ml) to each well of the 96-well plate, inc...

Embodiment 2

[0031] Embodiment 2: medication mode and result

[0032] (1) Medication alone

[0033] The detected cell lines were treated with different concentrations of fluvastatin, simvastatin, and abiraterone acetate for 72 hours, and the results were as follows: Figure 2-Figure 4 .

[0034] (2) Combined medication

[0035] Application of a kind of statin and abiraterone acetate joint treatment of above various cell lines for 72 hours, changes in cell viability as shown in the figure ( Figure 5-Figure 10 ), the concentration of each drug in combination is 1 / 2 of the single drug.

Embodiment 3

[0036] Embodiment 3: result analysis

[0037] After being treated with simvastatin or fluvastatin alone for 72 hours, the viability curves of various cells are as follows: figure 2 and image 3 , all kinds of cells showed decreased cell viability as the concentration of simvastatin or fluvastatin increased, among which Neuro2A in neuroblastoma and T98G in glioma were more sensitive to simvastatin.

[0038] After being treated with abiraterone acetate alone for 72h, the viability curves of various cells are as follows: Figure 4 , all kinds of cells showed that the cell viability decreased with the increase of the concentration of abiraterone acetate, among which SH-SY5Y in neuroblastoma and T98G in glioma were more sensitive to simvastatin. See Table 1 for the IC50 of the half-inhibitory dose of each cell line drug treatment.

[0039] After 72 hours of combined administration, although the concentration of each drug is 1 / 2 of that of single administration, the inhibition o...

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Abstract

The invention relates to a drug composition for treating neuroblastoma and neuroglioma and application thereof. The composition contains statin and Abiraterone acetate or its salt and is preferably acomposition prepared from simvastatin or Fluvastatin and Abiraterone acetate. The composition can have a remarkable synergistic effect on the aspect of treatment of neuroblastoma and neuroglioma.

Description

technical field [0001] The invention relates to the field of tumor (cancer) treatment, in particular to a composition of statin and abiraterone acetate and its application for treating neuroblastoma and glioma. Background technique [0002] Neuroblastoma (NB) is a malignant tumor originating from the embryonic sympathetic nervous system with unknown etiology. NB accounts for 8-10% of pediatric tumors, but accounts for 15% of pediatric cancer deaths. The 5-year survival rate of low-risk NB patients can reach 80-90%, but despite various treatments, the 5-year survival rate of high-risk NB patients is less than 50%. Approximately 40% of NB patients are high risk, who do not respond to first-line therapy or relapse within 2 years. Therefore, it is urgently needed clinically to find effective means of diagnosis and treatment, and to discover new therapeutic targets. [0003] Glioma (hereinafter referred to as glioma) is the most common brain tumor, which is characterized by ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61P25/00A61P35/00A61K31/58A61K31/366A61K31/405
CPCA61K45/06A61P35/00A61K31/366A61K31/405A61K31/58A61K2300/00
Inventor 王阳程传东方圣文
Owner 王阳
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