Corticotropin preparation method

A technology of corticotropin and ethanol aqueous solution, which is applied in the field of biomedicine, can solve the problems of low corticotropin content, long production cycle, cumbersome process, etc., and achieve the effect of complete extraction, low production cost and high content

Active Publication Date: 2019-03-05
SICHUAN XIELI PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a preparation method of corticotropin, which solves the problems in the prior art that the con

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] preparation of corticotropin

[0039] The mixer used in this embodiment is Supor soybean milk machine (JS30-230 type), and the centrifuge is Hunan Xiangli CTL550 type.

[0040] Preparation: Take 50g of dried anterior pituitary granules and crush them into solid powder with a mixer (25g of solid particles each time, stir for 3-5 minutes), and set aside for later use.

[0041] Activated D-101 macroporous resin, alcohol washing, water washing, acid washing, water washing, alkali washing, water washing, until the lotion is neutral and clear, soak in deionized water for use.

[0042] 1. Extraction: put the ground dry powder of anterior pituitary gland into a 3L three-necked bottle, connect a thermometer, add 1L of 1M / L HOAc aqueous solution, stir and wet and mix well, add dropwise 50% H 2 SO 4 The pH value of the solution adjustment system was 2.0. The reaction flask was placed on an oil bath, stirred and heated to make the internal temperature reach 80°C, maintained the ...

Embodiment 2

[0047] preparation of corticotropin

[0048] The equipment and preparation steps used in this example are the same as those in Example 1.

[0049] 1. Extraction: put the ground dry powder of the anterior pituitary gland into a 3L three-necked bottle, connect a thermometer, add 1L of 1M / L HOAc aqueous solution, stir, wet and mix well, add 50% H dropwise 2 SO 4 The pH value of the solution adjustment system is 2.5. The reaction flask was placed on an oil bath, stirred and heated to make the internal temperature reach 70°C, maintained the temperature, and kept stirring for 20 minutes for extraction. Then take out the oil bath, quench to 4°C, centrifuge (4000r / min, 5min), pour out the supernatant and combine to obtain the extract (stored at 15°C).

[0050] 2. Adsorption: Add 30g of activated D-101 macroporous resin to the extract obtained in the first step, and slowly stir and adsorb at 20°C for 10h, and the adsorption is completed. The adsorbed extract is filtered, and the ma...

Embodiment 3

[0054] preparation of corticotropin

[0055] The equipment and preparation steps used in this example are the same as those in Example 1.

[0056] 1. Extraction: put the ground dry powder of the anterior pituitary gland into a 3L three-necked bottle, connect a thermometer, add 1L of 1M / L HOAc aqueous solution, stir, wet and mix well, add 50% H dropwise 2 SO 4 The pH value of the solution adjustment system is 1. The reaction flask was placed on an oil bath, stirred and heated to make the internal temperature reach 75°C, maintained the temperature, and kept stirring for 20 minutes for extraction. Then the oil bath was taken out, quenched to 10°C, centrifuged (4000 r / min, 5min), the supernatant was poured out and combined to obtain the extract (stored at 10°C).

[0057] 2. Adsorption: Add 30g of activated D-101 macroporous resin to the extract obtained in the first step, and slowly stir and adsorb at 27°C for 15h, and the adsorption is completed. The adsorbed extract is filte...

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Abstract

The invention discloses a corticotropin preparation method, and solves the problems that corticotropin content is low, production cycle is long, operation is complicated, a process is tedious, and corticotropin cannot be detected by a physicochemical method in the prior art. The preparation method includes the steps: extracting anterior pituitary dry powder by acid solvents; adsorbing extracting solution by macroporous resin; performing gradient elution from low to high by ethanol water solution with different concentrations; collecting ethanol water solution eluent with the highest concentration; vaporizing the solvents; drying the solution to obtain corticotropin. The method is simple and convenient in operation, short in production cycle, low in production cost and suitable for industrial production. The corticotropin is completely extracted, low in loss in the purification process and high in yield, the corticotropin in a prepared finished product is high in content and can be detected by HPLC (high performance liquid chromatography), detection cost is effectively reduced, and detection cycle is shortened.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of corticotropin. Background technique [0002] Corticotropin (ACTH) is a polypeptide drug extracted from the anterior pituitary gland of pigs, cattle, sheep, etc., white or light yellow powder, odorless, hygroscopic, soluble in water, soluble in 70% Acetone and 70% ethanol are more stable in dry and weakly acidic solutions, but are easily deactivated in strong acids or alkalis. 100 ℃ heating stability. This product consists of 39 amino acid residues, and the species difference is only at positions 25-33. The fragment at positions 1-24 has full activity, and the part after the 24th amino acid only maintains the stability of the entire polypeptide structure. There is a high degree of α-helical structure in solution. Partially hydrolyzed by pepsin and still active. [0003] Corticotropin is a hormonal drug, and its clinical use is similar to...

Claims

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Application Information

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IPC IPC(8): C07K14/695C07K1/14
CPCC07K14/695
Inventor 赵亚囡唐天亢鹏
Owner SICHUAN XIELI PHARM CO LTD
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