Azotobacter chroococcum and identification method and application thereof

Abstract

The invention relates to azotobacter chroococcum and an identification method and application thereof. The gene sequence of azotobacter chroococcum 16S rDNA is as shown as SEQ ID NO.1; its bacterial strain is SINOPEC12, which is collected in the China Center for Type Culture Collection, with the collection number CCTCC NO:M2016110. The abundance of the bacterial strain in soil above oil and gas reservoirs has positive correlation with the concentration of floating gaseous hydrocarbon of the oil and gas reservoirs, and the bacterial strain can serve as oil and gas microorganism for indicating high-hydrocarbon-leakage-value zone above the oil and gas reservoirs; besides, the bacteria strain has high abundance in soil above the oil and gas reservoirs, and accordingly does not need multiplication culture; particularly, by modified primers, the conserved sequence of 16S rDNA of azotobacter chroococcum SINOPEC12 of soil can be amplified, the concentration of floating gaseous hydrocarbon of the oil and gas reservoirs can be determined accurately and effectively, and thus the defects of long cycle length of conventional biochemical detection and high requirements on purity of sample cultures are overcome.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a nitrogen-fixing bacterium rotundum and its identification method and application. Background technique [0002] The core of microbial exploration technology is the identification of oil and gas indicator microorganisms. Usually, the relative abundance of the target microorganisms studied in the exploration process is very low in the environment, so it is necessary to stimulate the rapid reproduction of hydrocarbon oxidizing bacteria in the oil and gas reservoir environment and non-oil and gas reservoir environment under the traditional specific culture conditions. Although the above method can obtain the quantity information of microorganisms, the quantity information is actually geometrically higher than the number of microorganisms in the in-situ environment, which inevitably conceals the difference in the number of microorganisms in oil and gas reservoirs and no...

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Problems solved by technology

[0004] At present, there are two common detection and identification methods for Azotobacter rotunda: 1. Physiological and biochemical identification, through the isolated pure bacteria, qualitative research on the microbial physiology and biochemistry, this method has a long cycle and puts forward higher requirements on the purity of the culture 2. After gene extraction, 16SrDNA sequencing is carried out. This method still needs to obtain pure cultures for whole gene extraction, and then use general 16SrDNA primers to obtain conserved sequences for sequencing

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