A kind of Streptomyces Nashville and its identification method and application

Abstract

The invention relates to a Streptomyces nasvierii and its identification method and application in the technical field of microorganisms. The gene sequence of the 16S rDNA of Streptomyces nashville is shown in SEQ ID NO.1, the strain is SINOPEC43 and the preservation number in China Center for Type Culture Collection is CCTCC NO: M2016114. The abundance of this strain in the soil above the oil and gas reservoir is positively correlated with the concentration of floating gaseous hydrocarbons in the oil and gas reservoir, and it can be used as an oil and gas microorganism to indicate the high value area of ​​hydrocarbon seepage above the oil and gas reservoir. At the same time, the abundance of this strain in the soil above oil and gas reservoirs is relatively high, and the 16S rDNA conserved sequence of Streptomyces nasvierii SINOPEC43 in the soil can be amplified through improved primers without amplification and cultivation, which can be accurate and efficient It can accurately judge the concentration of floating gaseous hydrocarbons in oil and gas reservoirs, which solves the shortcomings of long traditional physiological and biochemical detection cycles and high requirements for sample culture purity.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Streptomyces nasvierii and its identification method and application. Background technique [0002] The core of microbial exploration technology is the identification of oil and gas indicator microorganisms. Usually, the relative abundance of the target microorganisms studied in the exploration process is very low in the environment, so it is necessary to stimulate the rapid reproduction of hydrocarbon oxidizing bacteria in the oil and gas reservoir environment and non-oil and gas reservoir environment under the traditional specific culture conditions. Although the above method can obtain the quantity information of microorganisms, the quantity information is actually geometrically higher than the number of microorganisms in the in-situ environment, which inevitably conceals the difference in the number of microorganisms in oil and gas reservoirs and non-oil ...

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Problems solved by technology

[0004] At present, there are two common detection and identification methods for Streptomyces nasvierii: 1. Physiological and biochemical identification, through the isolated pure bacteria, qualitative research on the microbial physiology and biochemistry, this method has a long cycle and puts forward higher requirements on the purity of the culture Requirements; 2. 16S rDNA sequencing after gene extraction, this method still needs to obtain pure culture for whole gene extraction, and then use general 16S rDNA primers to obtain conserved sequences for sequencing

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