Rhodococcus equi strain and identifying method and application thereof

Abstract

The invention relates to a Rhodococcus equi strain and an identifying method and application thereof. A gene sequence of the Rhodococcus equi strain 16S rDNA is shown as SEQ ID NO. 1; the Rhodococcusequi strain as SINOPEC05 is collected in China Center for Type Culture Collection under CCTCC NO: M2016109. Abundance of the Rhodococcus equi strain in soil above oil-gas reservoirs is positively correlated with the concentration of floating gaseous hydrocarbons of the oil-gas reservoirs, and may act an oil-gas microorganism to indicate an region with hydrocarbon leak high value above the oil-gasreservoirs. In addition, the Rhodococcus equi strain has high abundance in the soil above oil-gas reservoirs; 16S rDNA conserved sequence of the Rhodococcus equi strain SNIOPEC05 in soil can be amplified through modified primers with no need for amplification culture; the concentration of floating gaseous hydrocarbons of the oil-gas reservoirs can be judged accurately and efficiently; the defect can be overcome that traditional physiological and biochemical detection has long cycle and has high requirement on sample culture purity.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a rhodococcus equi and its identification method and application. Background technique [0002] The core of microbial exploration technology is the identification of oil and gas indicator microorganisms. Usually, the relative abundance of the target microorganisms studied in the exploration process is very low in the environment, so it is necessary to stimulate the rapid reproduction of hydrocarbon oxidizing bacteria in the oil and gas reservoir environment and non-oil and gas reservoir environment under the traditional specific culture conditions. Although the above method can obtain the quantity information of microorganisms, the quantity information is actually geometrically higher than the number of microorganisms in the in-situ environment, which inevitably conceals the difference in the number of microorganisms in oil and gas reservoirs and non-oil and ga...

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Problems solved by technology

[0004] At present, there are two common detection and identification methods for Rhodococcus equi: 1. Physiological and biochemical identification, through the isolated pure bacteria, qualitative research on the microbial physiology and biochemistry, this method has a long cycle and puts forward higher requirements on the purity of the culture; 2. After gene extraction, 16S rDNA sequencing is performed. This method still needs to obtain pure cultures for whole gene extraction, and then use general 16S rDNA primers to obtain conserved sequences for sequencing

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