Solution and evaluation method for evaluating glucose metabolism through sialology method

Abstract

A solution and an evaluation method for evaluating glucose metabolism through a sialology method are disclosed, and the solution is prepared from 60-150 mM of Tris-HCl with pH of 7.6; 0.12-0.4% of blue chromogen; 1200-2500 IU / L of glucose oxidase; 120-250 IU / L of horseradish peroxidase and 600-1500 IU / L of an antioxidant. The evaluation method comprises the following steps: 1) collecting saliva;2) mixing the saliva with the solution; and 3), determining the glucose metabolism level of a body according to the color change of a mixed solution obtained after the saliva and the solution are mixed. According to the method disclosed by the invention, the glucose metabolism level of the body is evaluated by adopting the solution which generates color change after being reacted with the saliva,a lot of inconvenience of blood glucose detection is solved, and in addition, the method is visual, convenient and rapid, high in efficiency and high in accuracy. At the same time, the antioxidant isadded to the solution to extend the service life of the solution, waste is avoided, and cost is saved.

Description

technical field [0001] The invention relates to the technical fields of biology, veterinary medicine and medical research, in particular to a solution and an evaluation method for evaluating glucose metabolism through a salivary method. Background technique [0002] With the improvement of living standards, the proportion of people suffering from diabetes is increasing, and the detection of the body's glucose metabolism level is of great significance for preventing the occurrence of diabetes. [0003] Existing glucose detection methods include the previous detection method based on chemical glucose sensor and the detection method based on electrical glucose sensor, neither of which can provide a more convenient, quicker and lower-cost detection method. In addition to the above two methods, there is also the use of blood glucose detectors, which are costly and require high operation. [0004] The invention patent with the patent number 201480004302.4 discloses a saliva gluco...

AI Technical Summary

Problems solved by technology

However, the detection of blood sugar level needs to go through a complicated process of blood collection and testing, and blood collection itself is also a kind of harm to the body.

[0007] Existing evaluation of glucose metabolism level through saliva is to evaluate the glucose metabolism level by mixing saliva with the prepared reaction solution and observing the color depth to evaluate the glucose metabolism level. However, the chromogen substances currently used are mainly yellow, and the glucose metabolism can be evaluated by watching the depth of yellow. , but because saliva contains some yellow substances, especially in patients with oral bleeding, there are more yellow substances, which will affect the accuracy of the evaluation results; and the storage life of the existing reaction solutions is low