Preparation method and analgesic application of bauhinia brachycarpa extract and extractant thereof
A technology of the saddle leaf beetle and its extract, which is applied to the preparation of the saddle leaf beetle extract and its extract, and the application field of analgesics, and can solve the problems such as the absence of the saddle leaf beetle extract
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Embodiment 1
[0017] The preparation of the extract of the present invention and its extract:
[0018] Dry the above-ground part of S. saddleleaf plant (19.6 kg), crush it to 30 mesh, and extract it three times with 95% ethanol at room temperature, 100 L each time for 24 hours, combine the extracts, and concentrate the extracts under reduced pressure Obtain extract (2.4 kg). The obtained extract was suspended with 3.0 kg of water, and then distributed with 3 L of petroleum ether, ethyl acetate and n-butanol successively for 4 times; the extract of the corresponding parts and the remaining water phase were obtained.
Embodiment 2
[0020] The analgesic activity detection of the extract of the present invention and its extract:
[0021] An acetic acid writhing test was used to measure the analgesic activity of the extract of the present invention and the extract thereof. Kunming mice were half male and half female, weighing 18–22 g, fasted and watered 12 hours before the experiment, and randomly divided into control group, sample group, and positive drug group, with 8 mice in each group. Orally administered at a dose of 50–1000 mg / kg, the negative control group was given the same amount of normal saline, and the positive drug group was given aspirin. After 40 minutes of administration, each mouse was intraperitoneally injected with 0.6% glacial acetic acid solution 0.2 mL / 10g After injecting glacial acetic acid for 3-5 minutes, observe and record the number of writhing in mice within 15 minutes, and calculate the inhibitory rate of the test substance on the mice's acetic acid writhing according to the fol...
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