Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker for diagnosing severe asthma

A technology for severe asthma, which is applied in the field of molecular markers and molecular markers for the diagnosis of severe asthma, can solve uncontrollable problems, improve the quality of life and realize the effect of early diagnosis

Inactive Publication Date: 2019-03-08
CHANGZHOU NO 2 PEOPLES HOSPITAL
View PDF7 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (3) In the past year, according to the 4th or 5th level of asthma drug treatment recommended by the Global Initiative for Asthma Prevention (GINA), the control can be maintained or still can not be controlled

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker for diagnosing severe asthma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Screening Gene Markers Related to Asthma

[0060] 1. Sample collection

[0061] 3 ml of peripheral venous blood were collected from 3 normal persons, normal adult asthmatics, and severe adult asthmatics respectively. The patients all gave informed consent, and all the above specimens were obtained with the consent of the ethics committee.

[0062] Patient Inclusion Criteria:

[0063] Ordinary asthma patients meet the diagnostic criteria for bronchial asthma in the 2016 guidelines for the prevention and treatment of bronchial asthma; severe asthma meets the "Chinese Expert Consensus on the Diagnosis and Treatment of Severe Asthma"; all three groups of samples are taking any drugs in a short period of time; (4) Age > 18 years old .

[0064] Patient Exclusion Criteria:

[0065] Patients with co-infection, pulmonary embolism, chronic bronchitis, tuberculosis and blood system diseases; patients with abnormal liver function.

[0066] 2. RNA sample preparation an...

Embodiment 2

[0077] Example 2 QPCR sequencing to verify the differential expression of the ENSG00000258698 gene

[0078] 1. According to the detection results of high-throughput sequencing, the ENSG00000258698 gene was selected for large-sample QPCR verification. According to the method in Example 1, blood samples were collected from 45 normal asthma patients, 38 severe asthma patients, and 40 normal subjects.

[0079] 2. RNA extraction

[0080] Total RNA was extracted using the RNA extraction kit from Promega, and the specific steps are detailed in the instruction manual.

[0081] 3. Reverse transcription

[0082] The FastQμant cDNA First Strand Synthesis Kit of TIANGEN was used for reverse transcription. For details, please refer to the instruction manual.

[0083] 4. QPCR detection

[0084] 4.1 Primer design

[0085] QPCR amplification primers were designed according to the sequences of the gene encoding ENSG00000258698 and the GAPDH gene, and were synthesized by Shanghai Sangon Bi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a molecular marker for diagnosing severe asthma. The molecular marker is ENSG00000258698. The invention discloses that the expression of the ENSG00000258698 is up-regulated insevere asthma patients; the expression level of the ENSG00000258698 is detected to judge whether subjects have the severe asthma or not, so that early-period molecular diagnosis of the severe asthma is realized.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a molecular marker for severe asthma diagnosis, specifically the molecular marker is ENSG00000258698. Background technique [0002] Bronchial Asthma (Bronchial Asthma) is a common chronic inflammatory disease of the respiratory tract. There are about 300 million patients worldwide, which can be accumulated in all age groups. It is one of the health problems facing the world. It also imposes a severe economic burden on the country and individuals due to higher rates of disease, decreased daily activities, and increased utilization of medical resources. Inhaled glucocorticoids combined with long-acting beta 2 Receptor agonist treatment, most patients with bronchial asthma, especially mild and moderate patients, are treated well, but clinically, about 5% to 10% of asthma patients still cannot get well controlled symptoms after standardized treatment, and they need higher doses Severe asthm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883A61K45/00A61P11/06
CPCC12Q1/6883A61K45/00A61P11/06C12Q2600/158
Inventor 张倩毛正道钱艳
Owner CHANGZHOU NO 2 PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com