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Production method for gathering gamma-aminobutyric acid and flavonoid substances in sprouted salicornia europaea through combination salt stress

A technology of aminobutyric acid and production method, which is applied in the field of salicornia resource utilization, can solve the problems of little research on the utilization of salicornia buds, achieve good health care function and medicinal value, remarkable effect, and high-efficiency enrichment Effect

Inactive Publication Date: 2019-03-15
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on Salicornia is more focused on the nutrients of the plant, and there is little research on the utilization of Salicornia buds

Method used

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  • Production method for gathering gamma-aminobutyric acid and flavonoid substances in sprouted salicornia europaea through combination salt stress
  • Production method for gathering gamma-aminobutyric acid and flavonoid substances in sprouted salicornia europaea through combination salt stress

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Effect test

Embodiment 1

[0032] Germination under salt stress

[0033] Select plump and uniform Salicornia seeds, wash them with deionized water, remove suspended grains and impurities, sterilize with 1% sodium hypochlorite for 30 minutes, wash them with deionized water, and soak them in deionized water at a temperature of 25°C 8h. The seeds were placed in a culture box covered with a layer of gauze, evenly distributed, and cultured at 24°C under light conditions for 48 hours, and the light was turned off once every 12 hours, and the seeds were sprayed with 7.5g / L sodium chloride culture solution (pH 8.0) every 8 hours , keep it moist.

Embodiment 2

[0035] joint stress germination

[0036] Take salicornia germinated for 48 hours and grow uniformly, treat it with 7.5g / L sodium chloride and 5mmol / L calcium chloride for 96 hours, spray the mixed culture solution every 8 hours during the germination period, and take samples every 24 hours. After the buds are taken out, wash them with deionized water first, absorb the moisture on the surface with absorbent paper, and then dry them at 80-90°C for 24 hours. The collected Salicornia buds were stored in a refrigerator at 4°C. The obtained Salicornia buds were removed and ground, and the content of γ-aminobutyric acid was determined by high performance liquid chromatography, and the content of flavonoids was determined by the sodium nitrite-aluminum nitrate method, which was repeated 5 times.

Embodiment 3

[0038] Determination of γ-aminobutyric acid and flavonoids content:

[0039] (1) Determination of γ-aminobutyric acid content in salicornia buds by high performance liquid chromatography: Weigh 0.2 g of germinated salicornia dry powder and dissolve it in 5 mL of trichloroacetic acid solution with a mass fraction of 8%, shake in a water bath at 40 °C for 2 h , centrifuged, and the supernatant was filtered through a 0.45 μm filter membrane for the determination of GABA content. GABA was determined by pre-column OPA (phthalaldehyde) derivatized reversed-phase high-performance liquid chromatography to detect GABA. High-performance liquid chromatography HPLC (Agilent 1290), the detector wavelength is 338nm, the chromatographic column is a C18ODS reversed-phase column (250mm × 4.6mm, 5 μ m), the chromatographic column temperature is 25 ° C; the mobile phase is 0.1mol / L phosphate buffer (pH The value is 6.0) 52%, methanol 46%, tetrahydrofuran 2%, filter through 0.22 μm microporous m...

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Abstract

The invention relates to a production method for gathering gamma-aminobutyric acid and flavonoid substances in sprouted salicornia europaea through combination salt stress. The method comprises the steps that full and even salicornia europaea seeds are selected and cultured through sodium chloride salt stress for 48 h, so that the seeds adapt to the salt environment, by adding calcium chloride, combination stress sprouting is conducted, and accordingly the gamma-aminobutyric acid and flavonoid substances are gathered. The method has the advantages that the technology is simple, the productioncost is low, and the content of the gamma-aminobutyric acid and flavonoid substances is increased at the same time. The method is suitable for salicornia europaea bud production health care food-gradegamma-aminobutyric acid and flavonoid substances and can bring the economic benefit to salicornia europaea planting on the saline-alkali soil.

Description

technical field [0001] The invention belongs to the technical field of salicornia resource utilization, and in particular relates to a production method for enriching gamma-aminobutyric acid and flavonoids in germinated Salicornia germinated by combining salt stress. Background technique [0002] Salicornia Bigelivii Torr, also known as sea asparagus, sea beans, sea caterpillars, etc., belongs to the genus Salicornia in the family Chenopodiaceae, and belongs to the fleshy euhalophyte, which generally grows in saline-alkali land, salt lakes and beach areas. Salicornia has high nutritional value and is a good pollution-free green health food. It is known as "plant seafood" and "sea ginseng". The tender stems of Salicornia are rich in 18 kinds of amino acids, of which the content of amino acids necessary for the human body accounts for as high as 36.59%, which is very close to the high-quality protein standard (40%) given by FAO / WHO. The content of vitamins and minerals in Sal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06
CPCA01G7/06
Inventor 余晓红王杰王笃军李凤伟商曰玲
Owner YANCHENG INST OF TECH
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