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Bacillus subtilis strains for preparing fermented feather meal, and application of Bacillus subtilis strains

A technology of Bacillus subtilis and feather powder, which is applied in the fields of fermentation, preparation and application of organic fertilizers, and can solve problems such as high feed fish meal prices, practitioners' troubles, and unstable feed digestibility

Inactive Publication Date: 2019-03-26
AGRI TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In recent years, due to the high price of feed fish meal, feather meal converted from waste feathers is one of the industry-locked substitutes. However, the quality of hydrolyzed feather meal is currently unstable, resulting in unstable feed digestibility. considerable trouble

Method used

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  • Bacillus subtilis strains for preparing fermented feather meal, and application of Bacillus subtilis strains
  • Bacillus subtilis strains for preparing fermented feather meal, and application of Bacillus subtilis strains
  • Bacillus subtilis strains for preparing fermented feather meal, and application of Bacillus subtilis strains

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0048] The source and isolation and purification of preparation example 1 bacterial strain

[0049] The novel strain in this case, code-named Bacillus subtilis 531-7, was isolated from compost, and then compared with feather decomposition efficiency, and deposited in the biological resources preservation and research of the Food Industry Development Institute in Hsinchu, Taiwan, China Center, the number is BCRC 910793, and the deposit date is August 9, 2017; it is also deposited in the China Center for Type Culture Collection, the deposit number is CCTCC NO: M 2017495, and the deposit date is September 06, 2017. The following is a three-stage screening method for strains:

[0050] Stage 1: Comparison of Casein Decomposition Power

[0051] Take 1 gram (g) of compost and add 9 milliliters (mL) of sterile water, treat it in a water bath at 80°C for 20 minutes, or directly (room temperature) dilute it to 0.01 times and 0.001 times, and then spread it on the basic salt culture wit...

preparation example 2

[0056] Preparation example 2 bacterial strain basic physiological characteristic test

[0057] 1. The basic characteristics of the strain:

[0058] Appearance of Bacillus subtilis 531-7 strain: Gram-positive bacteria, the size of a single cell is about 0.4 μm to 0.6 μm×1.0 μm to 1.6 μm, and the top of the cell is semicircular and single or short chain. Spores are formed at the top of the cells, and the spores are oval or round, with a size of about 0.6 μm to 0.7 μm×1.0 μm to 1.6 μm. Forespores can be seen in electron micrographs of Bacillus subtilis 531-7 strain. When the Bacillus subtilis 531-7 bacterial strain of the present invention grows in LA medium, the initial stage (24 hours) bacterium colony shape is circular, white, the colony that bulge is formed on the surface, the bacterium colony with irregular edge or edge is smooth and tidy; Colony after 3 to 4 days The surface is dry and wrinkled.

[0059] 2. pH value measurement

[0060] The 3-day and 6-day feather mediu...

preparation example 3

[0063] Preparation Example 3 strain identification method

[0064] 1. BIOLOG strain identification and carbon source utilization capacity determination

[0065] (1) Measure the bacterial classification of bacterial strain of the present invention with BIOLOG identification system, compare the obtained numerical value with the database of BIOLOG company, if the OD of 71 kinds of carbon sources of this bacterial strain and 23 kinds of chemical sensitivity tests 595 When the similarity between the value and the strain value in the database reaches more than 0.50, the proximate identification ID of the measured strain is given. MicroStation provided by BIOLOG TM As a result of system software analysis, it was known that its phenotype had the highest similarity with the Bacillus subtilis strain, with a similarity of 0.64 and a possible probability of 0.77. Therefore, the strain was named Bacillus subtilis 531-7 (Bacillus subtilis 531-7).

[0066](2) Available carbon source and ch...

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Abstract

The invention relates to Bacillus subtilis strains for preparing fermented feather meal, and an application of Bacillus subtilis strains. An object of the present invention is to improve the hydrolyzed feather powder by adding Bacillus subtilis to the hydrolyzed feather powder for 1 days and increasing the digestibility of the feather powder with pepsin. Another object is to provide a Bacillus subtilis strain comprising a protease and a keratinase, which can be used in animal feed additives to enhance nutritional value. Strains of Bacillus subtilis 531 to 7 were isolated from the compost soiland compared by the degradability and enzyme activity test of feathers to confirm commercial value. Strains of Bacillus subtilis 531-7 were aligned by 16S rDNA sequencing and gyrB gene sequencing to confirm their novelty.

Description

technical field [0001] The present invention relates to a novel strain of Bacillus subtilis isolated from compost for the production of feather meal and protease containing keratinase activity. The invention also relates to a preparation method for reprocessing the hydrolyzed feather meal. Background technique [0002] The main protein in feathers is composed of insoluble structural protein-keratin. Keratin contains a high amount of hydrophobic disulfide bonds and hydrogen bonds, and is not easily decomposed by general proteolytic enzymes. Animals themselves lack such proteolytic enzymes, so keratin cannot be directly used by poultry and livestock. [0003] The method for hydrolyzing feather powder in the prior art is mainly realized by utilizing steam to hydrolyze feathers. Specifically as Figure 7 As shown, after the wet feathers are dehydrated (step 301), the feathers are input into the refining furnace, and the feathers are uniformly heated and stirred after injecting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12N9/50C05F1/00C05F11/08C05F17/00C12R1/125
CPCA23K10/12A23K10/18A23K10/26C05F1/005C12N1/20C12N9/50C12P1/04C05F17/20C12N1/205C12R2001/125C05F11/08Y02W30/40Y02A40/20
Inventor 罗致逑林良怡陈淑娟
Owner AGRI TECH RES INST
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