Application of valproic acid in preparation of anti-bacterial and anti-inflammatory drugs

An antibacterial and anti-inflammatory technology, valproic acid, applied in the preparation of antibacterial and anti-inflammatory drugs, new uses of valproic acid, new drug applications, can solve the problems that have not yet been reported on the application of valproic acid anti-bacterial and anti-inflammatory drugs

Inactive Publication Date: 2019-03-29
GENERAL HOSPITAL OF PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] At present, according to the prior art, valproic acid is mainly used in the preparation of drugs such as anti-tumor, treatment of epilepsy and bipola...

Method used

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  • Application of valproic acid in preparation of anti-bacterial and anti-inflammatory drugs
  • Application of valproic acid in preparation of anti-bacterial and anti-inflammatory drugs
  • Application of valproic acid in preparation of anti-bacterial and anti-inflammatory drugs

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Embodiment Construction

[0025] The following examples are provided to further illustrate but not limit the present invention.

[0026] 1. Materials and methods:

[0027] 1.1 Bacteria and cells

[0028] Fusobacterium nucleatum (F. nucleatum, Fn, ATCC 25586) was obtained from the frozen bacterial strain bank of the Key Laboratory of Oral Tissue Regeneration in Shandong Province.

[0029] Gingival fibroblasts (HGF): isolated from 6 volunteer patients aged 18-30 who underwent extraction of impacted teeth.

[0030] 1.2 Reagents

[0031] Defibrinated sheep blood (Haibo Bio Company, Qingdao, Shandong, China), PBS (Soleibao, Beijing, China), hemin-vitamin K1 (Haibo Bio Company, Qingdao, Shandong, China), brain Heart infusion blood culture medium (Suolaibao, Beijing, China), BHI liquid medium (Suolaibao, Beijing, China), type I collagenase (Suolaibao, Beijing, China), DispaseII dispase (Invitrogen, Carlsbad, CA, USA), intracellular reactive oxygen species detection kit (Bebo, Shanghai), valproic acid (Sel...

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Abstract

The invention discloses application of valproic acid in preparation of anti-bacterial and anti-inflammatory drugs. By performing RNA-Seq transcriptome analysis on human cells infected by bacteria, onbasis of co-expression pathway and drug genome enrichment analysis methods, a list of candidate drugs with anti-bacterial and anti-inflammatory effects are obtained; and then, several marketed drugs with the anti-bacterial and anti-inflammatory effects, as well as the valproic acid of which the anti-bacterial and anti-inflammatory effects have not been found before, are found out. Thus, the valproic acid can be used for preparing anti-bacterial and anti-inflammatory drugs.

Description

technical field [0001] The invention relates to a new application of medicine, in particular to a new application of valproic acid, specifically its application in the preparation of antibacterial and anti-inflammatory drugs, and belongs to the field of biomedicine. Background technique [0002] Valproic acid (valproic acid), a selective inhibitor of HDAC, also inhibits the proteasomal degradation of HDAC2, is used to treat epilepsy and bipolar disorder, and to prevent migraine. [0003] Valproic acid directly inhibits histone deacetylases through different pathways, with an IC50 of 0.4mM for HDAC1. In cultured cells, valproic acid acts like the histone deacetylase inhibitor trichostatin, causing deacetylation of histones. Valproic acid also activates transcription from different exogenous and endogenous promoters. In vertebrate embryos, valproic acid and trichostatin have very similar teratogenic effects without activating transcription (see Phiel, Christopher J., et al."...

Claims

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Application Information

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IPC IPC(8): A61K31/19A61K45/06A61P31/04A61P29/00
CPCA61K31/19A61K45/06A61P29/00A61P31/04A61K2300/00
Inventor 何昆仑贾志龙冯强康文燕王卫东石金龙赵晓静贾倩刘春蕾
Owner GENERAL HOSPITAL OF PLA
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