Method for preparing arginine-rich high-protein organic fertilizer by two-step fermentation process through using composite strain

A technology of compound bacteria and arginine, applied in the field of organic fertilizer, can solve the problems of low added value of products, difficulty in removal, waste of resources, etc., and achieve the effects of enhancing plant root development, improving salt stress resistance, and increasing protein content

Inactive Publication Date: 2019-03-29
SHANGHAI NONGLE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical method is mainly to remove anti-nutritional factors by heating, trypsin, etc. are sensitive to heat, but other inhibitory factors cannot be removed by heating, and heating will also destroy other nutrients; chemical methods are often not used, because chemical methods treat There will be a lot of chemical substances remaining in the process, resulting in difficulty in removal and increased cost. The biological method uses microorganisms to ferment defatted soybean meal, but the beany smell of the soybean meal fermentation liquid obtained by this method has not been well resolved
Peanut meal is currently mainly used for feed, and the added value of the product is low, resulting in a serious waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for preparing a high-protein organic fertilizer rich in arginine by a two-step fermentation method of compound strains, the specific steps are as follows:

[0031](1) Preparation of fermentation medium: Hemp meal, soybean meal and rice bran meal were crushed to 80 mesh respectively, mixed evenly according to the mass ratio of 5:4:1, and prepared into a mixture; molasses yeast fermentation waste liquid was added to the mixture to make the fermentation culture The moisture content of the base reaches 55%;

[0032] (2) preparation of mixed strain A: the mass ratio of mixed strain A is Bacillus subtilis: Aspergillus oryzae=1:2; (3) preparation of mixed strain B: the mass ratio of mixed strain B is pseudobacterium utilis Silk yeast: Lactobacillus bulgaricus=1:2;

[0033] (4) Add mixed strain A to the fermentation medium, its inoculum size is 4% (w / v), 8 layers of gauze are sealed and fermented for 24 hours, the fermentation temperature is 28 ° C, and the pre-fermen...

Embodiment 2

[0039] A method for preparing a high-protein organic fertilizer rich in arginine by a two-step fermentation method of compound strains, the specific steps are as follows:

[0040] (1) Preparation of fermentation medium: Sesame meal, peanut meal and soybean meal were crushed to 90 mesh respectively, mixed evenly according to the mass ratio of 4:5:2, and prepared into a mixture; molasses yeast fermentation waste liquid was added to the mixture to make the fermentation medium The moisture content reaches 58%;

[0041] (2) Preparation of mixed strain A: the mass ratio of mixed strain A is Bacillus subtilis: Aspergillus oryzae=2:3;

[0042] (3) Preparation of mixed strain B: the mass ratio of mixed strain B is Candida utilis: Lactobacillus bulgaricus=2:3;

[0043] (4) Add mixed strain A to the fermentation medium, its inoculum size is 5% (w / v), 8 layers of gauze are sealed and fermented for 26 hours, the fermentation temperature is 30°C, and the pre-fermentation liquid 1 is obtain...

Embodiment 3

[0049] A method for preparing a high-protein organic fertilizer rich in arginine by a two-step fermentation method of compound strains, the specific steps are as follows:

[0050] (1) Preparation of fermentation medium: Hemp meal, sesame meal and peanut meal were crushed to 100 mesh respectively, mixed evenly according to the mass ratio of 3:3:3, and prepared into a mixture; molasses yeast fermentation waste liquid was added to the mixture to ferment The moisture content of the medium reaches 60%;

[0051] (2) Preparation of mixed strain A: the mass ratio of mixed strain A is Bacillus subtilis: Aspergillus oryzae=3:4;

[0052] (3) Preparation of mixed strain B: the mass ratio of mixed strain B is Candida utilis: Lactobacillus bulgaricus=3:4;

[0053] (4) Add mixed strain A to the fermentation medium, its inoculum size is 6% (w / v), 8 layers of gauze are sealed and fermented for 28 hours, the fermentation temperature is 32 ° C, and the pre-fermentation liquid 1 is obtained;

...

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PUM

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Abstract

The invention discloses a method for preparing an arginine-rich high-protein organic fertilizer by a two-step fermentation process through using a composite strain. The method comprises the followingsteps: (1) respectively crushing plant dregs and plant meal, and uniformly mixing the crushed plant dregs and the crushed plant meal in proportion to prepare a mixture; (2) adding a molasses fermentation waste liquid to the mixture prepared in step (1) to prepare a fermentation medium; (3) inoculating the fermentation medium prepared in step (2) with a mixed strain A, and performing mixing fermentation at 28-32 DEG C for 24-28 h to obtain a pre-fermentation solution 1; (4) inoculating the pre-fermentation solution 1 with a mixed strain B, and carrying out mixing fermentation at 28-32 DEG C for24-48 h to obtain a post-fermentation solution 2; and (5) drying and crushing the post-fermentation solution 2 to obtain the arginine-rich high-protein organic fertilizer. The method has the advantages of safe process, convenience in preparation, and suitableness for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of organic fertilizers, in particular to a method for preparing high-protein organic fertilizers rich in arginine by mixing strains and two-step fermentation in a culture medium. Background technique [0002] The annual output of yeast in the world is more than 3 million tons. The production of yeast is mainly based on sugarcane molasses and beet molasses. Furfural, furfural can be degraded by acclimatized anaerobic sludge, but the degradation performance of dark caramel compounds is very poor. Caramel compounds exist in yeast wastewater, which makes the color of yeast wastewater darker. At the same time, melanin, phenols, caramel, macromolecular organic matter, inorganic salts and SO in wastewater 4 2- The concentration is relatively high, and the wastewater is rich in inorganic trace elements such as nitrogen, phosphorus, potassium, calcium, iron, and organic substances such as humic acid and fulvic acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F5/00C05F17/00
CPCC05F5/002C05F17/50C05F5/008C05F11/08Y02W30/40Y02A40/20
Inventor 于秋生韩粉丽冯镇泰何剑飞张红艳郝祥蕊於慧利
Owner SHANGHAI NONGLE BIOLOGICAL PROD
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