Preparation method of efficient growth-promoting bacterial fertilizer adopting waste jujube branches as nutrition medium

Abstract

The invention discloses a preparation method of efficient growth-promoting bacterial fertilizer adopting waste jujube branches as a nutrition medium. The preparation method comprises the following steps: MX26 strains, KDZ12 strains, LZJ3 strains and LZJ12 strains, which are separated and purified from rhizosphere soil, of growth-promoting bacteria are activated and then inoculated into a liquid culture medium to be subjected to shake culture on a shaking table for 12 h at 28 DEG C and 150 r / min, and I-level seed liquid is obtained; 4% of the I-level seed liquid is transferred into a bacterialbasic culture medium to be subjected to amplification culture for 15 h at 28 DEG C and 150 r / min, after culture, by adjusting bacterial liquid OD600, the bacterium amount reaches about 1.5*10<8> cfu / mL, and II-level seed liquid is obtained; all the II-level seed liquid are evenly mixed according to the volume ratio of 1:1:1:1 to obtain a flora; and the flora is inoculated according to the inoculating amount of 15%, a fermentation culture medium is made by adopting the smashed jujube branches as a nutrition source, after the flora is inoculated, the fermentation culture medium is placed in theenvironment of 28 DEG C to carry out constant-temperature static fermentation culture for 5-6 d, and the microbial fertilizer is obtained.

Description

technical field [0001] The invention relates to the field of bacterial fertilizer production, in particular to a method for preparing a high-efficiency growth-promoting bacterial fertilizer using discarded jujube branches as a nutrient matrix. Background technique [0002] At present, there are few microbial agents that can be applied to saline-alkali soil. One of the reasons is that the particularity of saline-alkali soil environment makes it difficult for some acid-producing bacteria to grow in it. The other is that saline-alkali soil is an extreme environment. Not enough effective strains could be isolated. However, due to the differences in the saline-alkali soil itself, the few bacterial agents studied can only be applied in a very small range. Contents of the invention [0003] In order to solve the above problems, the invention provides a method for preparing a high-efficiency growth-promoting bacterial fertilizer using discarded jujube branches as a nutrient subst...

AI Technical Summary

Problems solved by technology

[0002] At present, there are few microbial agents that can be applied to saline-alkali soil. One of the reasons is that the particularity of saline-alkali soil environment makes it difficult for some acid-producing bacteria to grow in it. The other is that saline-alkali soil is an extreme environment. Not enough effective strains could be isolated

However, due to the differences in the saline-alkali soil itself, the few bacterial agents that have been researched can only be applied in a very small range.

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