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Forest musk growth trait microsatellite site and early selection method

A technology for microsatellite loci and growth traits, applied in the field of microsatellite markers, can solve the problems of low selection accuracy, long cycle, incomplete match, etc., and achieve the effects of improving selection strength, high accuracy, and accelerating the breeding process.

Inactive Publication Date: 2019-03-29
四川养麝研究所
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for selection of the growth traits of forest deer musk deer, which is easily affected by various factors, and the phenotype and its genotype are not completely consistent, so that the selection accuracy is low and the cycle is long. Microsatellite locus and its primers for selection of growth traits of musk deer in early life and molecular genetic marker selection method for selection of growth traits (adult body weight) of musk deer

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  • Forest musk growth trait microsatellite site and early selection method
  • Forest musk growth trait microsatellite site and early selection method

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Embodiment 1

[0034] Example 1 Selection of growth traits of musk deer musk de musk musk deer parent group

[0035] (1) Design fluorescence-specific primers for the Mb18 and Mb37 sites of the linmus microsatellite: a pair of flanking sequences at both ends of the NCBI microsatellite site Mb18 (NCBI No. EF599337) and Mb37 site (NCBI No. EF599342) For specific primers, add HEX fluorescent label 5' to the upstream sequence of each pair of primers.

[0036] Mb18 site: the upstream primer is 5'-HEX-CTCCAGGCAAGAACACTG-3'; the downstream primer is 5'-GCAAGAAGTTATGCAATCAA-3';

[0037] Mb37 site: the upstream primer is 5'-HEX-TTGGGGTGAACTCAATCT-3'; the downstream primer is 5'-ATGGTATCTGACTCCAATAT-3'.

[0038] (2) Determination of growth traits of forest musk deer: Determination of fasting body weight (bw) of 1, 4 and 5 year old experimental musk deer, appearance indicators: chest circumference (cc), body length (bl) and body oblique length (bsl). The measurement method and each body size index ref...

Embodiment 2

[0049] Example 2 Selection of growth traits of the descendants of Dujiangyan musk musk deer

[0050] (1) Design fluorescence-specific primers for the Mb18 and Mb37 sites of the linmus microsatellite: a pair of flanking sequences at both ends of the NCBI microsatellite site Mb18 (NCBI No. EF599337) and Mb37 site (NCBI No. EF599342) For specific primers, add HEX fluorescent label 5' to the upstream sequence of each pair of primers.

[0051] Mb18 site: the upstream primer is 5'-HEX-CTCCAGGCAAGAACACTG-3'; the downstream primer is 5'-GCAAGAAGTTATGCAATCAA-3';

[0052] Mb37 site: the upstream primer is 5'-HEX-TTGGGGTGAACTCAATCT-3'; the downstream primer is 5'-ATGGTATCTGACTCCAATAT-3'.

[0053] (2) Determination of growth traits: Determination of the fasting body weight (bw) of the 1-year-old experimental musk deer, appearance indicators: chest circumference (cc), body length (bl) and body oblique length (bsl). The measurement method and each body size index refer to the method of Ya...

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Abstract

The invention discloses a forest musk growth trait microsatellite site and an early selection method. The microsatellite site is Mb18 or Mb37, wherein the NCBI number of the site Mb18 is EF599337, andthe NCBI number of the site Mb37 is EF599342. Primers for performing PCR amplification on the microsatellite site Mb18 or Mb37 are as shown in SEQ ID NO.1 to 4. Meanwhile, the invention also providesa forest musk growth trait microsatellite molecular marker selection method, which is completed by the step processes of performing polymorphic analysis through a PCR amplification product, selectinghigh-yield musk parent individuals and the like. The forest musk growth trait is selected according to the genotype, the precision is high and operation is simple; and the growth trait can start to be selected in the early period of the forest musk, generation interval is shortened, the forest musk breeding process is accelerated and each generation can shorten the selection time by about 12 months.

Description

technical field [0001] The invention belongs to the technical field of microsatellite markers, and in particular relates to a microsatellite locus for growth traits of Deer musk deer and an early selection method. Background technique [0002] Male growth of forest musk deer is an important economic trait in the production of forest musk deer, and it has been paid more and more attention in the breeding of forest musk deer. Since growth traits are economic traits that cannot be measured in vivo, the traditional selection methods for growth traits are mainly: (1) Select based on pedigree data, that is, select those with good blood relationship for seed use, and eliminate those with poor blood relationship; (2) According to the performance of siblings Selection, that is, to estimate the growth traits of breeding animals based on the carcass performance of full siblings or half-sibs; (3) Selection based on descendant performance, that is, to select the parents according to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2563/173C12Q2565/125
Inventor 蔡永华张明郑程丽杨营付文龙冯达勇
Owner 四川养麝研究所
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