High-resolution optical field microimaging system and method based on sub-pixel translation

Abstract

The invention discloses a high-resolution optical field microimaging system and method based on sub-pixel translation. The system comprises a microscope, a two-dimensional scanning galvanometer, a relay lens pair, a microlens array, an image sensor and a reconstruction module; the microscope is used for simplifying a target sample and imaging on a first image plane of the microscope; the two-dimensional scanning galvanometer is used for rotating an optical path angle in a frequency domain plane, and translating a first image plane in the sub-pixel; the relay lens pair is used for amplifying orshrinking the first image plane; the microlens array is used for modulating the beam with the preset angle to a target space location on a rear focal plane of the microlens array and obtaining multiple modulation images; the image sensor is used for recording multiple modulation images; and a reconstruction module for acquiring multiple modulation images recorded by the image sensor, and reconstructing a three-dimensional structure of the target sample through multiple modulation images. The system has the advantages of being simple in structure, low in cost, fast, and suitable for the livingcell observation.

Description

technical field [0001] The invention relates to the technical field of computational microscopy, in particular to a high-resolution light field microscopic imaging system based on sub-pixel translation. Background technique [0002] Three-dimensional imaging of biological samples is important for biological research, but cameras can only capture two-dimensional images at a time. Researchers have been working on effective 3D imaging methods. So far, related technologies mainly include the following three-dimensional microscopic imaging methods. [0003] The first method is to scan the sample axially. Common wide-field microscopes are capable of capturing two-dimensional sample slices in the focal plane. When the sample is moved through the axial motorized translation stage, different depths of sample slices can be focused. After moving the sample, the camera captures the image. We can stack these images and they effectively represent the real information about the specim...

AI Technical Summary

Problems solved by technology

However, this method has a non-negligible disadvantage: the process of moving the sample axially is very slow due to the required stability of the microscope stage

Therefore, the traditional light field has low spatial resolution and cannot accurately detect the sample

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