Rapid producing method for perineal pattern of root-knot nematode
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A technique for perineal patterns and root-knot nematodes, which is applied in the preparation, sampling, and measuring devices of test samples, can solve the problems of difficult operation, difficult operation, high difficulty of operation, etc., and achieve improved cutting process, easy cutting process, The effect of broad application prospects
Active Publication Date: 2019-04-02
宁波中盛产品检测有限公司 +1
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[0025] According to the literature, inserting a needle to remove the contents of the worm body is very difficult, and it is difficult to clean the contents
And, according to figure 2 The cutting surface is very small, and it is not easy to clean the contents. Moreover, this cutting method is also difficult to operate. It cannot be cut with one knife. It has to turn 90 degrees in the middle, which is very difficult to operate.
[0026] Therefore, the above-mentioned perineal pattern slicing methods have defects, and the operation is difficult, which is not conducive to the operation of laboratory appraisal personnel.
Method used
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Embodiment 1
[0059] Cut and make permanent slides with perineal patterns of female root-knot nematodes, the specific steps are as follows:
[0060] 1. Pick and transfer female insects: put the damaged roots in a petri dish with water, place them under a dissecting microscope, gently poke the surface of the root knot with tweezers or the tip of a dissecting needle, and the milky white smooth ball on the cross section is For female root-knot nematodes, use a No. 2 insect needle to gently dial the ball into the water, use a 200 microliter pipette, cut off the tip of the gun with a blade, and transfer more than one white female worm to the 7-10cm plastic petri dish for use.
[0061] 2. Cutting (for the first time): under a dissecting microscope magnified 10 times, use an insect needle to move the female in the above water drop to the outside of the water drop, and then use a No. 11 scalpel to follow the Figure 4 Use a No. 11 scalpel to cut the first cut, and then use an insect needle to tran...
Embodiment 2
[0066] Example 2 Comparison of different perineal patterns sheeting methods
[0067] Carry out root-knot nematode perineal pattern making respectively according to the method of embodiment 1, Liu Weizhi method and Gerber and Taylor, choose 3 experimenters, in 10 days, each make 5 pieces of root-knot nematode perineal pattern with three kinds of methods every day, statistics Production time, success rate, calculate the average production time and draw a production time curve, see the results Figure 5 . The success rate data are shown in Table 1.
[0068] The failures mainly include skin damage during cleaning, or cutting patterns during cutting, etc.
[0069] The production time only calculates the average time of successful production, and the failure is not counted.
[0070] Table 1 Comparison of the success rate data of different perineal pattern production methods
[0071]
[0072] In Table 1, A, B, and C represent different experimenters.
[0073] Figure 5 The r...
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Abstract
The invention discloses a rapid producing method for a perineal pattern of the root-knot nematode. The method comprises the following steps: step one, selecting and transferring a female nematode; step two, cutting down the one-fifth to half of the nematode body with the complete perineal pattern of the tail of the female nematode obliquely by using a No.11 scalper and then transferring the partial nematode body with the perineal pattern to a lactic acid solution with the mass grade of 60%; step three, carrying out cleaning; to be specific, enabling the incision to be placed downwardly or facethe side, pressing the cuticular layer of the nematode body for once to five times by using an insect needle, releasing contents in the nematode body quickly, and cleaning the contents quickly for 10to 60 seconds; step four, carrying out cutting along the edge of the pattern by using the No.11 scalper to obtain a small cuticular layer containing the complete perineal pattern; and step five, making a permanent slide of the perineal pattern of the root-knot nematode. According to the invention, with the optimized cutting mode, the perineal pattern part is cut down once and the profile area islarge by oblique cutting; content cleaning is carried out directly on the large cuticular layer; and thus the operation becomes convenient. The time for implementing the method is shortened; the success rate is high; and the cuticular layer is protected from being damaged in cleaning.
Description
technical field [0001] The invention relates to a method for making permanent slides with perineal patterns of root-knot nematodes. Background technique [0002] With the integration of the global economy and the increasing international trade, the risk of alien quarantine pests being introduced into my country is becoming more and more serious. my country has become one of the countries most seriously threatened by alien quarantine pests in the world. Biological invasions have attracted the attention of governments all over the world due to their social, economic and ecological problems. These invasive species not only seriously threaten the ecosystem, accelerate species extinction, but also cause the loss of biodiversity. [0003] At present, there are 20 species (genera) of quarantine nematodes included in the list of quarantine pests for imported plants in my country, among which root-knot genus (non-Chinese species) is quarantine nematodes. Root-knot nematodes have the...
Claims
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