Rapid producing method for perineal pattern of root-knot nematode

Abstract

The invention discloses a rapid producing method for a perineal pattern of the root-knot nematode. The method comprises the following steps: step one, selecting and transferring a female nematode; step two, cutting down the one-fifth to half of the nematode body with the complete perineal pattern of the tail of the female nematode obliquely by using a No.11 scalper and then transferring the partial nematode body with the perineal pattern to a lactic acid solution with the mass grade of 60%; step three, carrying out cleaning; to be specific, enabling the incision to be placed downwardly or facethe side, pressing the cuticular layer of the nematode body for once to five times by using an insect needle, releasing contents in the nematode body quickly, and cleaning the contents quickly for 10to 60 seconds; step four, carrying out cutting along the edge of the pattern by using the No.11 scalper to obtain a small cuticular layer containing the complete perineal pattern; and step five, making a permanent slide of the perineal pattern of the root-knot nematode. According to the invention, with the optimized cutting mode, the perineal pattern part is cut down once and the profile area islarge by oblique cutting; content cleaning is carried out directly on the large cuticular layer; and thus the operation becomes convenient. The time for implementing the method is shortened; the success rate is high; and the cuticular layer is protected from being damaged in cleaning.

Description

technical field [0001] The invention relates to a method for making permanent slides with perineal patterns of root-knot nematodes. Background technique [0002] With the integration of the global economy and the increasing international trade, the risk of alien quarantine pests being introduced into my country is becoming more and more serious. my country has become one of the countries most seriously threatened by alien quarantine pests in the world. Biological invasions have attracted the attention of governments all over the world due to their social, economic and ecological problems. These invasive species not only seriously threaten the ecosystem, accelerate species extinction, but also cause the loss of biodiversity. [0003] At present, there are 20 species (genera) of quarantine nematodes included in the list of quarantine pests for imported plants in my country, among which root-knot genus (non-Chinese species) is quarantine nematodes. Root-knot nematodes have the...

AI Technical Summary

Problems solved by technology

[0025] According to the literature, inserting a needle to remove the contents of the worm body is very difficult, and it is difficult to clean the contents

And, according to figure 2 The cutting surface is very small, and it is not easy to clean the contents. Moreover, this cutting method is also difficult to operate. It cannot be cut with one knife. It has to turn 90 degrees in the middle, which is very difficult to operate.

[0026] Therefore, the above-mentioned perineal pattern slicing methods have defects, and the operation is difficult, which is not conducive to the operation of laboratory appraisal personnel.

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