Unlock instant, AI-driven research and patent intelligence for your innovation.

Control of coleopteran pests using RNA molecules

A molecular and nucleic acid molecule technology, applied in the field of pest control, can solve the problem of inability to provide coleopteran pest mortality and other problems

Inactive Publication Date: 2019-04-02
SYNGENTA PARTICIPATIONS AG
View PDF30 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These authors reported that even at more than 520ng / cm 2 Eight of the 26 target genes they tested also failed to provide experimentally significant coleopteran pest mortality at very high concentrations of iRNA (e.g., dsRNA)
Additionally, dsRNA molecules directed against target genes are known to produce strong RNAi effects in one insect species, such target genes may not be good targets for a different insect species

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Control of coleopteran pests using RNA molecules
  • Control of coleopteran pests using RNA molecules
  • Control of coleopteran pests using RNA molecules

Examples

Experimental program
Comparison scheme
Effect test

example

[0190] The present invention may be further described by reference to the following specific examples. These examples are provided for illustrative purposes only and are not intended to be limiting unless otherwise indicated. Example 1. Identification of RNAi gene targets in western corn root firefly beetle

example 1

[0190] The present invention may be further described by reference to the following specific examples. These examples are provided for illustrative purposes only and are not intended to be limiting unless otherwise indicated. Example 1. Identification of RNAi gene targets in western corn root firefly beetle

[0191] This example describes the cloning and sequencing of RNAi target genes and coding sequences from Rhizoctonia insects.

[0192] Preparation and Sequencing of Pyrosequencing Library of Western Maize Rhizome

[0193] Whole body neonatal Western corn rootworm (WCR )) Transcriptome sequencing. The resulting determined reads (i.e., short fragments of nucleic acid sequence) were trimmed and assembled into contigs using the MIRA assembler (see, e.g., Chevreux et al. 2004 Genome Res. 14:1147-1159, incorporated by reference here).

[0194] Identification of the most important target genes from Rhizoctonia genus

[0195] The assembled contigs were compared by BLAST to k...

example 2

[0204] Example 2.dsRNA (4 kinds of concentrations) against the activity of western corn root firefly beetle-DRC

[0205] This example describes the testing of the biological activity of dsRNAs of the invention against western corn root beetle (WCR).

[0206] Starting from 1 μg dsRNA / well, the above 30 dsRNA molecules were tested for toxicity against WCR in a 10-fold dilution series in laboratory bioassays. Bioassays were performed using the RNA-treated artificial diet method. Briefly, molten artificial feed modified from the feed of Marrone et al. 1985 (J. Econ. Entomol. [Journal of Economic Entomol] 78:290-293) was poured into each well of a 48-well plate and allowed to solidify . Synthetic dsRNA molecules were diluted to appropriate concentrations such that 20 μl of the solution was added to the surface of the feed in half of the wells of the 48-well plate, with final coverage concentrations of 1 μg, 0.1 μg, 0.01 μg and 0.001 μg per well. Add one or two WCR larvae to each...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed are double stranded RNA molecules that are toxic to coleopteran insects. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.

Description

[0001] sequence listing [0002] The Sequence Listing in ASCII text format is provided as an alternative to a paper copy, filed pursuant to 37 C.F.R. §1.821, named "81040_ST25.txt", 467 kilobytes in size, generated on June 22, 2017 and Submit via EFS-Web. This Sequence Listing is hereby incorporated by reference into this specification for its disclosure. technical field [0003] The present invention relates generally to the control of pests that cause damage to crop plants due to their feeding activities, and more particularly to the control of coleopteran pests by compositions comprising interfering RNA molecules. The invention further relates to compositions comprising interfering RNA molecules and to methods of using such compositions. Background technique [0004] Insect species in the Rhizopus genus (corn rootworm and cucumber beetle) are considered some of the most important pests to crop plants. For example, species of corn rootworm, including Western corn rootwo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/82C12N15/87C12N15/113C12N15/12C12N15/32A61K31/713A01C1/06A01P7/04A01N63/60
CPCA61K45/06C12N15/113C12N15/8218C12N15/8286C12N2310/14A61K31/713Y02A40/146A01N63/60A01N63/14A01C1/06C12N15/87
Inventor K·V·多诺霍Y·瑙德特P·菲尔德曼L·德拉格夫I·马莱特
Owner SYNGENTA PARTICIPATIONS AG