Astragalus polysaccharide and bio-extraction method

A technology for biological extraction and astragalus polysaccharide, applied in the field of polysaccharide extraction, can solve the problems of increased energy consumption in the extraction process, increased extraction cost, increased environmental burden, etc., and achieves the effects of protecting the environment, being easy to operate, and reducing subsequent processing work.

Inactive Publication Date: 2019-04-05
河北远征禾木药业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The commonly used extraction methods of astragalus polysaccharides are alkaline alcohol precipitation method, alkaline water extraction method, ultrasonic extraction method, high pressure, microwave extraction method, etc., which can increase the extraction amount of astragalus polysaccharides to a certain extent,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The present embodiment provides a biological extraction method of astragalus polysaccharides, comprising the following steps:

[0050] 1) Pass the pulverized Astragalus membranaceus through a 40-mesh sieve, add a buffer solution with a pH value of 4.8 at a ratio of 1:3, add a combined enzyme of cellulase, hemicellulase and pectinase, and Enzymolysis at 50°C for 2 hours;

[0051] Wherein, the added amounts of the cellulase, hemicellulase and pectinase are respectively 5‰, 5‰, and 2.5‰ of the weight of the astragalus;

[0052] 2) Inactivate the reaction solution after enzymolysis at 100°C for 15 minutes, add 6 times the amount of Astragalus in water and decoct it for 0.5 hours twice, filter to obtain the decoction solution and medicinal residues, combine the decoction solution, and put the The decoction is concentrated, sealed and stored at low temperature for later use;

[0053] 3) Dry the dregs until the moisture content is 45%, then inoculate the Bacillus subtilis an...

Embodiment 2

[0058] The present embodiment provides a biological extraction method of astragalus polysaccharides, comprising the following steps:

[0059] 1) Pass the pulverized Astragalus membranaceus through a 40-mesh sieve, add the buffer solution with a pH value of 4.5 at a ratio of 1:1.5 to the material-liquid ratio, add the combined enzyme of cellulase, hemicellulase and pectinase, and Enzymolysis at 55°C for 1 hour;

[0060] Wherein, the added amounts of the cellulase, hemicellulase and pectinase are respectively 7.5‰, 5‰, and 2.5‰ of the weight of the astragalus;

[0061] 2) Inactivate the reaction solution after enzymolysis at 100°C for 15 minutes, add 6 times the amount of Astragalus in water and decoct it for 0.5 hours twice, filter to obtain the decoction solution and medicinal residues, combine the decoction solution, and put the The decoction is concentrated, sealed and stored at low temperature for later use;

[0062] 3) Dry the dregs until the moisture content is 45%, the...

Embodiment 3

[0067] The present embodiment provides a biological extraction method of astragalus polysaccharides, comprising the following steps:

[0068] 1) Pass the pulverized Astragalus membranaceus through a 40-mesh sieve, add a buffer solution with a pH value of 5.5 at a ratio of 1:5, add a combined enzyme of cellulase, hemicellulase and pectinase, and Enzymolysis at 45°C for 3 hours;

[0069] Wherein, the added amounts of the cellulase, hemicellulase and pectinase are respectively 2.5‰, 2.5‰, and 5‰ of the weight of the astragalus;

[0070] 2) Inactivate the reaction solution after enzymolysis at 100°C for 15 minutes, add 6 times the amount of Astragalus in water and decoct it for 0.5 hours twice, filter to obtain the decoction solution and medicinal residues, combine the decoction solution, and put the The decoction is concentrated, sealed and stored at low temperature for later use;

[0071] 3) Dry the dregs until the moisture content is 45%, then inoculate the Bacillus subtilis ...

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Abstract

The invention relates to an astragalus polysaccharide and a bio-extraction method. The bio-extraction method comprises the steps that astragalus is crushed and screened, then three composite enzymes of cellulase, hemicellulase and pectinase are added for enzymolysis, then decoction is conducted, and decoction liquids are combined; bacillus subtilis and lactic acid bacteria are inoculated into decoction dregs for fermentation, decoction is conducted again after fermentation, all decoction liquids are combined, after concentration and alcohol precipitation, 95% ethyl alcohol is added for washing, and the astragalus polysaccharide is obtained after drying. Through the astragalus polysaccharide and a bio-extraction method, the yield of the astragalus polysaccharide can be greatly improved, theextraction of the astragalus is maximized by the specificity and high efficiency of the enzymes and the biological characteristic of biological fermentation, meanwhile, pollution caused by emission of acid-base organic solvents and the like is avoided, the last remaining dregs can be used as organic fertilizer, and environment protection in the production process is achieved.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to an astragalus polysaccharide and a biological extraction method thereof. Background technique [0002] Astragalus is the dried root of the leguminous plant Astragalus mongolica or Astragalus membranaceus. The main active ingredients are polysaccharides, flavonoids, saponins, amino acids, etc., which have the functions of solidifying Qi and raising Yang, promoting diuresis to reduce swelling, detoxifying and discharging pus. The astragalus polysaccharide contained in it is polymerized by galactose, glucose, rhamnose, arabinose, glucuronic acid and galacturonic acid. Studies have shown that astragalus polysaccharides have the properties of enhancing the body's immunity, regulating blood sugar, and anti-aging. [0003] Cellulose is a macromolecular polysaccharide composed of glucose, usually combined with hemicellulose, pectin, and lignin. Plant cell walls are co...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C12P39/00C12R1/125
CPCC12P19/04C12P19/14C12P39/00
Inventor 张沛杨国辉刘冬王德功李卓伟周岩田俊岭魏丽娟魏永辉孔瑞岗
Owner 河北远征禾木药业有限公司
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