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Method for calculating and calibrating material content and device

A technology of material content and calculation method, applied in the calculation of material content, calibration method and device field, can solve the problem of low accuracy rate, achieve the effect of eliminating calculation error and improving accuracy

Active Publication Date: 2019-04-05
EDAN INSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the embodiment of the present invention provides a method and device for calculating and calibrating substance content to solve the problem of low accuracy in calibration or calculation of substance content in the prior art

Method used

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  • Method for calculating and calibrating material content and device
  • Method for calculating and calibrating material content and device
  • Method for calculating and calibrating material content and device

Examples

Experimental program
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Embodiment 1

[0026] figure 1 It shows the implementation flow of the calculation method of the substance content provided by the first embodiment of the present invention, and is described in detail as follows:

[0027] In S101 , during the reaction process of the reaction solution in which the content of the target substance is unknown, signal values ​​representing the reaction process corresponding to multiple time values ​​are detected.

[0028] Optionally, in the embodiment of the present invention, the reaction solution containing the target substance but unknown content is poured into the cuvette, and during the reaction of the reaction solution, light of a specific frequency is emitted from one side of the cuvette, and the other side Measure scattered light intensity or absorbance. It can be understood that since scattered light intensity or absorbance is a signal detected by the tester during testing, both scattered light intensity and absorbance can be collectively referred to as...

Embodiment 2

[0048] As mentioned in the above embodiment S102, according to the corresponding relationship between the time value and the signal value, the regression equation is fitted. Due to the influence of environmental factors, there may be large errors in the signal values ​​detected at certain moments, and these errors may cause the fitted regression equation to fail to reflect the changing trend of the signal values ​​well, so in the embodiment of the present invention In , it is necessary to delete part of the data corresponding to the time values ​​with large errors, and use the signal values ​​corresponding to the remaining time values ​​to fit the regression equation. figure 2 The specific implementation process of S102 provided by Embodiment 1 of the present invention is shown, and the details are as follows:

[0049] In S201, fitting the corresponding relationship between the time value and the signal value to generate a first equation.

[0050] In the embodiment of the pr...

Embodiment 3

[0060] The embodiment of the present invention provides another implementation method for S102 in the first embodiment above, image 3 Another specific implementation process of S102 provided by Embodiment 1 of the present invention is shown, which is described in detail as follows:

[0061] In S301, normalization processing is performed on the signal value to generate a signal mapping value.

[0062] The embodiment of the present invention considers that the range of the signal value may be different in each detection process, so it is necessary to normalize the signal value of each detection, so that the subsequent calculated signal difference is more consistent , which is convenient for comparison with the preset corresponding relationship between signal difference and content.

[0063] Optionally, via the formula: Map the detected signal value to the interval [0, 1], where Y' i is the signal mapping value corresponding to the i-th moment value, y i is the signal value...

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Abstract

The invention is applicable to the technical field of biochemical detection, and provides a method for calculating and calibrating material content and a device. The method comprises the following steps: detecting signal values corresponding to a plurality of time values in a preset time period, and fitting a regression equation according to the correspondence relationship between the time valuesand the signal values to stably reflect the change trend of the signal values; substituting a time value in the preset time period as an independent variable into the regression equation; using a dependent variable calculated by the regression equation as a signal theoretical value corresponding to the time value; calculating the difference between two signal theoretical values corresponding to the preset time value according to the regression equation, and using the difference as a signal difference value; and determining the content of a target material according to the correspondence relationship between the signal difference value and the content, so as to eliminate the calculation error of the change amount of the signal value caused by inaccurate selection of the preset time value, thereby improving the calculation accuracy of the target material content.

Description

technical field [0001] The invention belongs to the technical field of biochemical detection, in particular to a method and device for calculating and calibrating substance content. Background technique [0002] At present, when detecting the content of a specific protein, immunoturbidimetry is often used. Taking antigen as an example, the specific principle is: when the specific antigen to be tested is combined with the specified specific antibody, the reaction solution becomes turbid, and the antibody When the content is fixed, the amount of change in turbidity of the reaction solution is positively correlated with the antigen content. By detecting the change in turbidity of the reaction solution and comparing it with a series of standard substances, the antigen content in the test sample can be calculated. Among them, the measurement of the turbidity of the reaction solution can be carried out by scattering turbidimetry or transmission turbidimetry, that is, at the begin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/49G01N21/31
CPCG01N21/31G01N21/49
Inventor 黎伟禧虞坤桥甘泉
Owner EDAN INSTR
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