A colorimetric assay for ascorbic acid based on degradable nanozymes

Abstract

The invention discloses an ascorbic acid colorimetric detection method based on biodegradability nano-enzyme. On the basis of a principle that the degradation function of enzyme catalysis and ascorbicacid for the enzyme is simulated on the basis of a hydroxyl nanowire is simulated, the method comprises the following steps that: preparing degradable nano-enzyme test paper sheets, preparing detection cards, carrying out a sample dripping operation, carrying out the color extraction of the detection cards in sample tanks and a contrast tank, correcting the color evaluation values of the detection cards in the sample tanks, combining the color correction evaluation value with an ascorbic acid fitting model, and detecting a to-be-detected sample which contains ascorbic acid concentration. By use of the method, the degradable nano-enzyme is used for constructing a vision detection card used for measuring the ascorbic acid, and the test paper sheet is replaced and reused to realize the quickmeasurement of the ascorbic acid. Compared with the prior art, the method has a low requirement on detection equipment, and a detection process can be finished only by the detection card and a smartphone.

Description

technical field [0001] The invention relates to the field of food detection, in particular to an ascorbic acid colorimetric detection method based on degradable nanozyme. Background technique [0002] Ascorbic acid (AA), also known as vitamin C, exists in various fruits, vegetables and animal feed, and is one of the most important vitamins for maintaining normal physiological functions of the human body. Ascorbic acid participates in many biological processes, such as the anti-oxidation of DNA and protein in the human body, and the scavenging of free radicals, which play a key role. Various studies have shown that the expression level of ascorbic acid is closely related to many diseases, such as scurvy, liver disease, arteriosclerosis, cardiovascular disease, cancer and so on. However, as an essential nutrient, ascorbic acid cannot be produced by the human body's own physiological system, and it must be supplemented through exogenous intake to maintain normal human body fun...

AI Technical Summary

Problems solved by technology

Although some methods use nanozyme for colorimetric detection of ascorbic acid, most of them use the product catalyzed by ascorbic acid reduction nanozyme to achieve the detection purpose, and the sensitivity and selectivity are poor; Without instability, poor reproducibility, non-portability and other defects, it cannot meet the needs of on-site instant detection of ascorbic acid

Images