Technology for simultaneous determination of sulfonamides and chloramphenicols in honey

Abstract

The invention discloses a technology for simultaneous determination of sulfonamides and chloramphenicols in honey. A method comprises the following steps: 0.2% of acetonitrile formate is taken as an extracting agent; through purification treatment of a novel small column (Oasis PRi ME HLB column), a flowing phase is made up in volume and passes through a membrane and then is put on a machine to betested; and the sulfonamides and the chloramphenicols in the honey can be extracted at the same time. The chloramphenicols have a good linear relationship in a range of 0.1-10,0 ng / mL, and a correlation coefficient is larger than 0.991; and the sulfonamides have a good linear relationship in a range of 0.5-50.0 ng / mL. The minimum detection limit of a chloramphenicol sample is 0.1 [mu]g / kg, the detection limit of the sulfanilamides is 0.5 [mu]g / kg, the recovery rate ranges from 82.50% to 101.20%, and the relative standard deviation is 3.2%-4.0%. According to the technology for the simultaneousdetermination of the sulfanilamides and the chloramphenicols in the honey, the method is rapid, accurate and high in sensitivity, and is suitable for detection of the chloramphenicols and the sulfonamides in the honey.

Description

technical field [0001] The invention belongs to the technical field of biology and new medicine, and specifically relates to the technical field of veterinary medicine. Background technique [0002] Sulfonamide drugs are suitable for treating infectious diseases such as hemolytic streptococcus, meningococcus, and pneumococcus, and used as feed additives to prevent and treat infections such as staphylococcus and hemolytic streptococcus. Because of its long-lasting and significant effect, sulfa drugs are commonly used in bee breeding for disease prevention and treatment. However, unreasonable use will easily cause sulfa drugs to remain in honey and other agricultural products. After the drugs enter the human body through the food chain, they will cause diseases such as hematuria, crystalluria, and kidney damage, which will cause harm to human health. At the same time, the residues of sulfa drugs in honey It also creates obstacles to the honey trade. Many countries have estab...

AI Technical Summary

Problems solved by technology

However, unreasonable use will easily cause sulfa drugs to remain in honey and other agricultural products. After the drugs enter the human body through the food chain, they will cause diseases such as hematuria, crystalluria, and kidney damage, which will cause harm to human health. At the same time, the residues of sulfa drugs in honey also creates obstacles to the honey trade