Chimeric antigen receptor based on human mesothelin antibody, lentiviral expression vector and its application

A technology of chimeric antigen receptor and lentivirus, which is applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, virus/bacteriophage, receptor/cell surface antigen/cell surface determinant, etc. Solve the problems of unsatisfactory effect, exhaustion, T cell incompetence, etc., and achieve the effect of improving therapeutic effect, improving function and expansion, and efficient recognition
CN109608549BActive Publication Date: 2022-02-18THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
Publication Date
2022-02-18

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Abstract

The invention belongs to the field of biotechnology, and specifically relates to a chimeric antigen receptor based on human mesothelin antibody, a lentiviral expression vector and applications thereof. The chimeric antigen receptor consists of human CD8a molecular signal peptide, human mesothelin The single-chain antibody of corticosteroid, the flexible fragment of human CD8a molecule, the transmembrane region and intracellular region of human CD28 molecule, the intracellular region of human 41BB molecule, and the intracellular region of human CD3z molecule are sequentially connected in series. The chimeric antigen receptor prepared by the present invention can target mesothelin and improve the therapeutic effect of CAR-T cells.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a chimeric antigen receptor based on human mesothelin antibody, a lentivirus expression vector and applications thereof. Background technique

[0002] Mesothelin is highly expressed in a variety of tumors and is a good target for tumor therapy. Using transgenic technology, the chimeric antigen receptor (CAR) sequence that recognizes mesothelin is transfected into T cells, and CAR-T cells that specifically recognize mesothelin and kill tumor cells can be generated. CAR-T cell therapy has shown good results in hematological tumors, but the effect in solid tumors is unsatisfactory. The shortcomings of CAR-T cells in the treatment of solid tumors are related to the following three issues: 1. Whether the source of the recognition region of CAR-T cells is human or other species; Signals from the external recognition region are transmitted to the intracellular activation regio...

Claims

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