Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chimeric antigen receptor based on human mesothelin antibody, lentiviral expression vector and its application

A technology of chimeric antigen receptor and lentivirus, which is applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, virus/bacteriophage, receptor/cell surface antigen/cell surface determinant, etc. Solve the problems of unsatisfactory effect, exhaustion, T cell incompetence, etc., and achieve the effect of improving therapeutic effect, improving function and expansion, and efficient recognition

Active Publication Date: 2022-02-18
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CAR-T cell therapy shows good results in hematological tumors, but unsatisfactory results in solid tumors
The shortcomings of CAR-T cells in the treatment of solid tumors are related to the following three issues: 1. Whether the source of the recognition region of CAR-T cells is human or other species; Signals from the outer recognition region are transmitted to the intracellular activation region to initiate T cell killing; 3. Whether the activation of CAR will lead to rapid incompetence or even exhaustion of T cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chimeric antigen receptor based on human mesothelin antibody, lentiviral expression vector and its application
  • Chimeric antigen receptor based on human mesothelin antibody, lentiviral expression vector and its application
  • Chimeric antigen receptor based on human mesothelin antibody, lentiviral expression vector and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The experimental methods in the following examples are conventional methods unless otherwise specified, and the involved experimental reagents and materials are conventional biochemical reagents and materials unless otherwise specified.

[0020] 1. CAR Structure

[0021] The basic structure of meso-CAR consists of human CD8a molecule signal peptide (Leading signal), human mesothelin single-chain antibody (scFv), human CD8a molecule flexible fragment (CD8Hinge), human CD28 molecule transmembrane region and intracellular region (CD28) , human 41BB molecular intracellular region (41BB) and human CD3z molecular intracellular region (CD3z) are sequentially connected in series, and the structure formed in series is as follows figure 1 shown. The amino acid sequence of the meso-CAR structure is shown in SEQ ID NO.7.

[0022] Wherein, the amino acid sequence of human CD8a molecular signal peptide (Leading signal) is:

[0023] MALPVTALLLPLALLLLHAARP (SEQ ID NO.1)

[0024] Th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of biotechnology, and specifically relates to a chimeric antigen receptor based on human mesothelin antibody, a lentiviral expression vector and applications thereof. The chimeric antigen receptor consists of human CD8a molecular signal peptide, human mesothelin The single-chain antibody of corticosteroid, the flexible fragment of human CD8a molecule, the transmembrane region and intracellular region of human CD28 molecule, the intracellular region of human 41BB molecule, and the intracellular region of human CD3z molecule are sequentially connected in series. The chimeric antigen receptor prepared by the present invention can target mesothelin and improve the therapeutic effect of CAR-T cells.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a chimeric antigen receptor based on human mesothelin antibody, a lentivirus expression vector and applications thereof. Background technique [0002] Mesothelin is highly expressed in a variety of tumors and is a good target for tumor therapy. Using transgenic technology, the chimeric antigen receptor (CAR) sequence that recognizes mesothelin is transfected into T cells, and CAR-T cells that specifically recognize mesothelin and kill tumor cells can be generated. CAR-T cell therapy has shown good results in hematological tumors, but the effect in solid tumors is unsatisfactory. The shortcomings of CAR-T cells in the treatment of solid tumors are related to the following three issues: 1. Whether the source of the recognition region of CAR-T cells is human or other species; Signals from the external recognition region are transmitted to the intracellular activation regio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/867C12N7/01C12N5/10
CPCC12N7/00C12N15/86C07K14/7051C07K16/30C07K2319/33C07K2319/02C07K2317/622C12N2740/15043C12N2740/15021
Inventor 张毅李峰张震
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com