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Detection method and system for colorectal cancer

A colorectal cancer, target nucleic acid technology, applied in biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc., can solve the problems of low diagnostic value and large trauma of carcinoembryonic antigen

Active Publication Date: 2019-04-12
深圳微健康基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, carcinoembryonic antigen has little diagnostic value for early cases, and although colonoscopy is highly accurate, it is more traumatic

Method used

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  • Detection method and system for colorectal cancer
  • Detection method and system for colorectal cancer
  • Detection method and system for colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Screening and verification of biomarkers

[0050] Collected 193 stool samples from Southwest Hospital in Chongqing, China, including 98 colorectal cancer patient samples (68 male samples, 30 female samples) and 95 healthy control samples (49 male samples, 46 female samples) . We performed metagenomic detection on 52 colon cancer patient samples and 55 healthy control samples (from healthy family members with similar diet structure, living habits, and living environment) to detect colorectal cancer Differences in gut microbiota between patients and healthy people. In the process of detecting the above samples, we identified 30 microbial gene fragments related to colorectal cancer, of which 26 gene fragments were increased in colorectal cancer patient samples, and 4 gene fragments were increased in healthy control samples increased in (see figure 1 ). figure 2 The receiver operating characteristic (ROC) curve showed that the 30 candidate gene fragments show...

Embodiment 2

[0067] Example 2 Primer Screening

[0068] Specific primers were designed for the biomarker-specific sequences obtained through the screening in Example 1, and primer tests were performed on the specific primers designed for each specific sequence: that is, by ordinary PCR amplification, and the strips were detected by agarose gel electrophoresis. If the size of the target band is the same as expected and the band is single, it indicates that the specificity of the primer is good. After detection, it was found that the primers shown in Table 2 can be used to amplify the specific sequence screened in Example 1, the target band size is consistent with the expectation and the band is single, and the specificity of amplification is good.

[0069] Table 2 Specific primer sequences

[0070]

Embodiment 3

[0071] Embodiment 3 colorectal cancer detection application

[0072]This example is mainly based on the real-time fluorescent quantitative PCR technology to test the DNA of the fecal microbial flora of colorectal cancer patients and normal people, and the results shown are the results provided by the best primers provided in Example 2.

[0073] 1. Sample collection

[0074] Sampling swabs were used to collect the central parts of feces from 30 colorectal cancer patients and 30 normal people, and stored in the feces collection tube containing buffer solution. After tightening the tube cap, turn it upside down 5 or 6 times, and mix the preservation solution and The feces are well mixed.

[0075] Specimen storage and transportation: store at room temperature or refrigerated at 4°C. Specimens can be transported at room temperature for long-distance transport before refrigeration, and dry ice is required for long-distance transport of specimens after refrigeration.

[0076] 2. N...

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Abstract

The invention relates to the field of genetic test, in particular to a marker of colorectal cancer and an application of the marker. The marker comprises SEQID NO:1, SEQID NO:2 and SEQID NO:3. Besides, the invention further provides a detection method and system for colorectal cancer on people to be tested. The marker provided by the invention can realize fast detection of the colorectal cancer.

Description

technical field [0001] The present invention relates to the field of gene detection, in particular to a colorectal cancer marker and its application, in particular to a colorectal cancer marker, a primer pair, and a method and system for detecting colorectal cancer in a subject. Background technique [0002] Colorectal cancer is one of the most common malignant tumors of the digestive tract, with approximately 1 million new cases and approximately 500,000 deaths worldwide each year. Colorectal cancer (colorectal cancer, CRC) ranks third in incidence and fourth in mortality among various cancers, and nearly 55% of the cases occur in developed countries. The incidence rate is low in people younger than 40 years old, but the incidence rate gradually increases with age. It is generally believed that Westernization of dietary habits will increase the incidence of CRC, but the incidence in the United States and other high-income countries has remained stable or even decreased, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/689C12Q1/6851C12Q1/06C12N15/11
CPCC12Q1/6851C12Q1/6886C12Q1/689C12Q2531/113C12Q2545/101
Inventor 栗东芳戴文魁李寅虎周潜李嘉琪
Owner 深圳微健康基因科技有限公司
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