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A screening method for salt-tolerant and ethylene-insensitive mutants of peanuts

An ethylene-insensitive and mutant technology, applied in the biological field, can solve problems such as the lack of relevant research on the mechanism of salt tolerance

Active Publication Date: 2022-04-08
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the previous study, 165 peanut salt-tolerant candidate germplasms were obtained through in vitro mutagenesis and salt-tolerance screening, but no relevant research has been conducted on the salt-tolerance mechanism

Method used

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  • A screening method for salt-tolerant and ethylene-insensitive mutants of peanuts
  • A screening method for salt-tolerant and ethylene-insensitive mutants of peanuts
  • A screening method for salt-tolerant and ethylene-insensitive mutants of peanuts

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Embodiment Construction

[0026] The present invention will be described in detail below in combination with specific embodiments.

[0027] 1. Experimental materials

[0028] 165 peanut salt-tolerant candidate germplasm obtained through in vitro mutagenesis and salt-tolerant directional screening, and the mutagenized parental control variety "Huayu 22".

[0029] 2. Experimental method

[0030] The present invention comprises following specific experimental steps:

[0031] 1. Determination of screening concentration of ethephon

[0032] Wash off the soil adhering to the sand surface, rinse it twice with clean water, put it in a beaker, sterilize it under high temperature and high pressure (121°C, 15min), and then dry it in the air. Add 250mL of ethephon solution to 1000mL of sand and stir evenly, set the concentration gradient of ethephon to 1, 3, 5, 7, 10 mg·L -1 . Add sand to the beaker to the 500mL mark, place 10 peanut seeds on the surface of the sand, add sand to the 1000mL mark, and seal it w...

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Abstract

The invention discloses a screening method for salt-tolerant ethylene-insensitive mutants of peanuts, which uses a sandy soil cultivation method; primary screening of ethephon; secondary screening of aminocyclopropanecarboxylic acid (ACC), a precursor of ethylene synthesis; secondary screening of NaCl; and dark cultivation And measure the hypocotyl length, and finally screen the peanut salt-tolerant ethylene-insensitive mutant. The beneficial effect of the invention is that the method is simple and can effectively screen peanut salt-tolerant ethylene mutants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a screening method for salt-tolerant ethylene-insensitive mutants of peanuts. Background technique [0002] Peanut is an important oil crop and economic crop in my country, and plays an important role in agriculture and even the entire national economy. The situation of land salinization in my country is very severe, and cultivating new varieties of salt-tolerant crops is the most economical and effective way to utilize saline soil. The genetic basis of cultivated peanuts is narrow, and there is a lack of highly salt-tolerant germplasm in the existing resources. The creation and screening of salt-tolerant mutants and new salt-tolerant germplasm through mutagenesis technology are of great significance for the breeding of salt-tolerant peanuts. It is an effective strategy to improve the utilization efficiency of saline-alkali land. In the previous study, 165 peanut salt-tolerant candid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04
CPCA01H1/04
Inventor 乔利仙周文杰孙明明刘文平赵方贵朱虹隋炯明王晶珊
Owner QINGDAO AGRI UNIV