A kind of method for cultivating hepatitis E virus in vitro

A hepatitis E virus, in vitro culture technology, applied in the directions of viruses, viruses/phages, biochemical equipment and methods, etc., can solve the problem that cannot use cancer cell line HEV vaccine research and development and production, cannot be stably continuously subcultured, cannot be real. Reaction-related symptoms, etc.
CN109652384BActive Publication Date: 2021-10-26KUNMING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
KUNMING UNIV OF SCI & TECH
Publication Date
2021-10-26

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Abstract

The invention discloses a method for culturing hepatitis E virus in vitro. The method uses MDCK cells to culture hepatitis E virus in vitro, and uses RT-nPCR, PT-qPCR and immune confocal methods to confirm that the hepatitis E virus can MDCK cells are replicated in large quantities, and can be stably passed down for more than 10 generations. This method has made a major breakthrough in the in vitro culture of hepatitis E virus. In order to further study the biological and immunological characteristics of hepatitis E virus, HEV infection and pathogenicity The mechanism has laid a good foundation.
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Description

technical field

[0001] The invention belongs to the field of biotechnology and relates to a new method for successfully culturing and continuously replicating hepatitis E virus in vitro. Background technique

[0002] Hepatitis E Virus (HEV) is a viral hepatitis pathogen transmitted through the intestinal tract, which can infect humans and various animals across species. HEV is a single-stranded positive-sense RNA virus. Current studies have found that there are mainly 8 genotypes (HEV-1---HEV-8), and the genotype 4 HEV strain (HEV-4) is mainly prevalent in China. It has been confirmed that the transmission route of HEV is mainly the fecal-oral route, and it can also be transmitted by other routes, such as blood transmission, contact transmission, vertical transmission, and organ transplantation.

[0003] HEV infection is generally an acute self-limiting infection, and patients can recover after 4-6 weeks of self-detoxification. However, immunodeficiency patients and the el...

Claims

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