Signature sequences, labeled primers and identification method of thin-shell hickory nut species Sumner

Abstract

The invention relates to multiple pairs of high-specificity signature sequences of a thin-shell hickory nut species Sumner, molecular specific labeled primers and a method capable of rapidly and accurately identifying the thin-shell hickory nut species Sumner. The sequences of a molecular specific labeled primer group are as shown in the follow: 1), an upstream primer: 5'-AAGGGGCTTTGCAGATCCTC-3',and a downstream prier: 5'-ATCCAAGGCAGCTCTTCCAC-3'; 2), an upstream primer: 5'-TGGCATGCCCAACTTGATAGT-3', and a downstream primer: 5'-GCATGACCCTCGTTCGAGAA-3'; (3), an upstream primer: 5'-AGAGCCGATTCTTCCATGGT-3', and downstream primer: 5'-ACCATTTGGATCTTCCCCATCA-3'. The molecular specific labeled primers have the advantages that rapid and early identification can be conducted on the thin-shell hickory nut species Sumner, the method is simple, rapid and accurate, and the molecular specific labeled primers are irreplaceable molecular means for identifying the species of thin-shell hickory nuts through phenomenal characteristics.

Description

technical field [0001] The invention relates to a characteristic sequence of the pecan variety Sumner, a molecular specific marker primer combination and a method for specifically identifying the pecan variety Sumner by using the molecular specific marker primer combination. Background technique [0002] Carya illinoënsis (Wangenh.) K.Koch) is the most economically valuable tree species in the genus Carya illinoënsis (Wangenh.) K. Koch; Carya illinoënsis is a typical outcross plant. The variety configuration of pecans is one of the main factors affecting its yield; the United States is one of the origins of pecans, and it is also the central production area. Over the years, the United States has selected and named about 1,000 cultivars; Walnut has a history of more than 100 years, and there are dozens of varieties commonly used in production at present. Years of production practice have proved that the vast subtropical regions of my country are the suitable growth areas for th...

AI Technical Summary

Problems solved by technology

[0003] However, the main problem faced by the existing pecan production areas in my country is the low and unstable yield. There are many reasons for this phenomenon, one of which is the lack of clear genetic relationship analysis of the existing introduced varieties. Coupled with the confusion of the naming of some hybrid offspring, it is difficult to carry out effective parent selection and rational allocation, and it is not convenient for variety identification, promotion, communication and cultivation of new varieties. Therefore, efforts have been made to develop some stable and specific DNA varieties at the molecular level. Fingerprint marking is a scientific way to realize accurate and rapid identification of pecan varieties;

[0004] Some molecular marker methods have been reported at home and abroad for the identification and kinship analysis of pecan varieties. The SSR molecular marker method is better, but these old detection methods are cumbersome and the results are unstable.

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