Method for preparing micro vesicle based on myristic acid and imidazole derivative non-covalent complex

A technology of myristic acid and imidazole compounds is applied in the field of preparing microvesicles based on non-covalent complexes of myristic acid and imidazole derivatives, which can solve the problem of poor stability of ordered aggregates of microvesicles, and preparation of instruments and equipment. It has the advantages of convenient industrial production and promotion, low cost, and easy operation.

Active Publication Date: 2019-04-26
HUBEI POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to address the problems of expensive preparation equipment and materials, high cost, cumbersome preparation procedures, and poor stability of the prepared microvesicle ordered aggregates in the current preparation method for the preparation of ordered microvesicle aggregates. It is easy to be damaged during use, and provides a method for preparing microvesicles based on the non-covalent complex of myristic acid and imidazole derivatives

Method used

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  • Method for preparing micro vesicle based on myristic acid and imidazole derivative non-covalent complex
  • Method for preparing micro vesicle based on myristic acid and imidazole derivative non-covalent complex
  • Method for preparing micro vesicle based on myristic acid and imidazole derivative non-covalent complex

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Embodiment 1

[0029] A method for preparing microvesicles based on a non-covalent complex of myristic acid and imidazole derivatives in this embodiment, comprises the following steps in sequence:

[0030] (1) Preparation of non-covalent complexes of myristic acid and N-methylimidazole

[0031] Weigh 14.02g of myristic acid, dissolve it in an appropriate amount of absolute ethanol, place it in an ice-water bath, stir and protect it under nitrogen, slowly add 8.21g of N-methylimidazole compound dropwise to a 500mL round bottom flask, and keep stirring , under the condition of nitrogen protection, slowly add the ethanol solution of myristic acid dropwise, and stir the reaction for 24 hours under nitrogen atmosphere; -Methylimidazole non-covalent compound crude product;

[0032] (2) Recrystallization and purification of non-covalent complexes of myristic acid and N-methylimidazole

[0033] Dissolve the crude myristic acid and N-methylimidazole complex in an appropriate amount of acetone under...

Embodiment 2

[0037] (1) Preparation of non-covalent complexes of myristic acid and 4-methylimidazole

[0038] Weigh 19.06g of myristic acid, dissolve it in an appropriate amount of absolute ethanol, place it in an ice-water bath, stir, and protect it under nitrogen; slowly add 10.25g of 4-methylimidazole compound dropwise to a 500mL round bottom flask, and keep stirring, Under the condition of nitrogen protection, the ethanol solution of myristic acid was slowly added dropwise, and the reaction was stirred for 18 hours under nitrogen atmosphere; Methylimidazole non-covalent complex crude product;

[0039] (2) Recrystallization and purification of non-covalent complexes of myristic acid and 4-methylimidazole

[0040] The obtained myristic acid and 4-methylimidazole non-covalent complex crude product was dissolved in an appropriate amount of acetone under heating conditions, and then 300ml of organic solvent acetonitrile was added, and the solution mixture was placed at room temperature for...

Embodiment 3

[0044] (1) Preparation of non-covalent complexes of myristic acid and 5-chloro-1-methylimidazole

[0045] Weigh 15.20g of myristic acid, dissolve it in an appropriate amount of absolute ethanol, place it in an ice-water bath, stir and protect it under nitrogen, and slowly add 16.22g of 5-chloro-1-methylimidazole compound dropwise into a 500mL round bottom flask , under the conditions of keeping stirring and nitrogen protection, slowly drop the ethanol solution of myristic acid, and stir and react under nitrogen atmosphere for 36 hours; Myristic acid and 5-chloro-1-methylimidazole non-covalent complex crude product;

[0046] (2) Recrystallization and purification of non-covalent complexes of myristic acid and 5-chloro-1-methylimidazole

[0047] Dissolve the crude myristic acid and 5-chloro-1-methylimidazole non-covalent complex in an appropriate amount of acetone under heating, then add 100ml of acetonitrile, cool slowly, and let stand for 2 hours, the solution precipitates T...

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Abstract

The invention relates to a method for preparing a micro vesicle based on a myristic acid and imidazole derivative non-covalent complex. The method sequentially comprises the following steps: (1), preparing the myristic acid and imidazole derivative non-covalent complex; (2), recrystallizing and purifying a crude product of the myristic acid and imidazole derivative non-covalent complex; (3), preparing the micro vesicle through a fine product of the myristic acid and imidazole derivative non-covalent complex. The method has the advantages that raw materials are easy to obtain, the cost is low,the preparation method is simple and convenient to operation, a prepared product has a stable micro vesicle structure, an obvious Maltese cross shape can be seen through polarizing microscope characterization, and the product has relatively good flowability; a micro vesicle system formed with the method has relatively good stability and relatively high vesicle concentration, can be applied to various aspects, such as cell biological membrane simulation, drug delivery, drug sustained release, micro-nanometer reactors, preparation of nanometer materials and the like, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of ordered aggregate materials of soft matter surfactants, and in particular relates to a method for preparing microvesicles based on a non-covalent compound of myristic acid and imidazole derivatives. Background technique [0002] Molecularly ordered aggregate structures exist widely in nature, such as cell membranes of animals and plants, double-helix DNA in life, bacteria, viruses, etc. all belong to ordered molecular aggregates. Ordered molecular aggregates can be constructed through the ordered aggregation of surfactants. It has been found that through the ratio of surfactants to various solvents, as the concentration of surfactants changes, spherical micelles, rod micelles, Various orderly aggregates of micelles such as lamellar and columnar micelles, structural expansion can form various lyotropic liquid crystal aggregate structures, among which flexible worm-like micelles, double-layered, multi-layered and oth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F17/00B01F17/32C09K23/00C09K23/32
CPCC09K23/017
Inventor 马福民颜玉影李文陈跃程雅倩
Owner HUBEI POLYTECHNIC UNIV
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