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Embryo culture solution containing oviduct-derived exosome and embryo culture method

An embryo culture solution and embryo culture technology, which is applied to the embryo culture solution containing fallopian tube-derived exosomes and the field of in vitro embryo culture, can solve the problems that no fallopian tube-derived exosomes have been seen, and achieve the effect of promoting in vitro development

Active Publication Date: 2019-04-26
XI AN JIAOTONG UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0004] At present, there is no report on the application of oviduct-derived exosomes in embryo culture fluid

Method used

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  • Embryo culture solution containing oviduct-derived exosome and embryo culture method

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Embodiment Construction

[0026] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, which are only used to explain the present invention, rather than limit the protection scope of the present invention.

[0027] There are a large number of exosomes in the fallopian tube fluid, and the fallopian tube-derived exosomes contain RNA, protein, lipid and other substances, which enable the fallopian tube-derived exosomes to play a role in the embryonic and maternal environmental signal communication. The present invention improves the efficiency of in vitro culture of embryos by extracting oviduct-derived exosomes and utilizing the embryo culture solution containing the exosomes for embryo culture.

[0028] 1. Source and preparation of reagents

[0029] Pituitary follicle-stimulating hormone (PMSG) and chorionic gonadotropin (HCG) for injection were purchased from Ningbo Sansheng Pharmaceutical Co., Ltd. Sterile water for injection was purcha...

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Abstract

The invention discloses an embryo culture solution containing an oviduct-derived exosome and an embryo culture method: extracting the oviduct-derived exosome from oviduct fluid, adding the oviduct-derived exosome to an embryo base culture solution to obtain the embryo culture solution containing the oviduct-derived embryo exosome, wherein the concentration of the oviduct-derived embryo exosome inthe embryo culture solution is 6.0 to 7.0*10<8> cells / mL; and the embryo of an animal such as a rabbit is transferred into the embryo culture solution containing the oviduct-derived exosome to culture. According to the invention, the function of the internal oviduct regulatory factor in the in vitro culture environment is obtained by adding the oviduct-derived exosomes, which is beneficial to thedevelopment of the embryo in the in vitro environment and improves the in vitro culture efficiency of the embryo.

Description

technical field [0001] The invention relates to in vitro embryo culture, in particular to an embryo culture solution containing oviduct-derived exosomes and an embryo culture method. Background technique [0002] As an important experimental animal, rabbits are widely used in the research of human diseases. Especially in the research of cardiovascular disease, lipid metabolism and embryonic development, using rabbit as a model has played an important research value. In addition, rabbits occupy an important position in people's lives as economic animals or pets. With the development of gene editing technology, a variety of genetically engineered rabbits have been born one after another. However, the assisted reproductive technology of rabbits is not mature compared with other species. So far, there is no commercial rabbit embryo culture medium at home and abroad. The in vitro culture of rabbit embryos is at a relatively low level, which greatly limits the production effici...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
CPCC12N5/0604C12N2500/84
Inventor 刘恩岐屈鹏祥贾林莹李璐
Owner XI AN JIAOTONG UNIV
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