Nanoporous thin film, gene sequencing device and preparation method of nanoporous thin film
A nanoporous film and nanopore technology, applied in the field of gene sequencing, can solve the problems of few effective data points and inability to distinguish bases, etc.
Active Publication Date: 2019-04-26
BOE TECH GRP CO LTD
View PDF6 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
It can be seen that the main problem of the existing nanopore sensors to realize single base recognition is that the speed of DNA molecules passing through the nanopore is too fast, resulting in too few effective data points acquired by the existing patch clamp or other signal acquisition systems, and cannot Each base is resolved based on the signal that blocks the current
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0041] In order to make the purpose, technical solutions and advantages of the embodiments of the present disclosure clearer, the technical solutions of the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings of the embodiments of the present disclosure. Apparently, the described embodiments are some of the embodiments of the present disclosure, not all of them. Based on the described embodiments of the present disclosure, all other embodiments obtained by persons of ordinary skill in the art without creative effort fall within the protection scope of the present disclosure.
[0042] Unless otherwise defined, the technical terms or scientific terms used in the present disclosure shall have the usual meanings understood by those skilled in the art to which the present disclosure belongs. "First", "second" and similar words used in the present disclosure do not indicate any order, quantity or importance, but are o...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides a nanoporous thin film, a gene sequencing device and a preparation method of the nanoporous thin film. The nanoporous thin film comprises a substrate, a thin film body which isarranged on one surface of the substrate and a nano porous channel which penetrates through the thin film body, wherein a through hole corresponding to the nano porous channel is formed in the substrate; the nano porous channel is provided with a detecting part which is used for allowing a single nucleic acid molecule to pass and is capable of changing the moving direction of the nucleic acid molecule so as to reduce the moving speed of the nucleic acid molecule. According to the nano porous thin film provided by the invention, the nano porous channel is provided with the detecting part whichjust allows the single nucleic acid molecule to pass and the detecting part is capable of changing the moving direction of the nucleic acid molecule so as to reduce the moving speed of the nucleic acid molecule, and the speed that the nucleic acid molecule passes through the detecting part is slower, so that data volume of an obtained detecting signal can be increased, and the accuracy of gene sequencing can be further increased.
Description
technical field [0001] The present disclosure relates to the technical field of gene sequencing, in particular, to a nanoporous film, a gene sequencing device using the nanoporous film, and a method for preparing the nanoporous film. Background technique [0002] Since the 1970s, people have developed three generations of DNA sequencing technology. The first-generation DNA sequencing technology was based on the Sanger method, and it took 15 years to complete the Human Genome Project, with a direct cost of about US$3 billion. The second-generation DNA sequencing technology is characterized by high throughput, and it takes only one week and costs less than US$1 million to complete the sequencing of the human genome. In recent years, DNA single-molecule detection and analysis based on solid-state nanopore devices is considered to be one of the most promising technical routes to realize the third generation of rapid and low-cost human gene sequencing, and has become a hot spot ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): C12Q1/6869
CPCC12Q1/6869
Inventor 董水浪王利忠刘清召赵磊卢鑫泓党宁
Owner BOE TECH GRP CO LTD