HLA sequencing peak graph identification method
A map recognition and sequencing technology, applied in the field of peak map recognition of HLA next-generation sequencing, can solve problems such as inability to accurately identify sequences, and achieve the effects of long time-consuming, high recognition accuracy, and strong ease of use.
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[0030] Example HLA sequencing peak map recognition method
[0031] The steps are as follows (the flow chart is as follows figure 1 shown):
[0032] (1) Constructing a electropherogram recognition model:
[0033] ①Collect a large amount of artificially identified data and import it into the preprocessing module of the electropherogram identification system, such as image 3 shown;
[0034] ②Preprocess the imported HLA electropherogram data, complete the information extraction of the binary ab1 file, the original sequence comparison, sequence segmentation and dislocation repair;
[0035] The "preprocessing" includes multiple processing of electropherogram data (flow chart as figure 2 Shown): electropherogram reading, sequence comparison, dislocation repair, data arrangement; in the electropherogram reading stage, the data identification and reading of off-machine sequencing files are mainly completed (converting binary electropherogram data files into ordinary text data ,S...
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