A kind of sea cucumber saponin nano-liposome for injection administration and preparation method thereof

A technology of nano-liposomes and sea cucumber saponins, which can be used in liposome delivery, pharmaceutical formulations, medical preparations of non-active ingredients, etc., and can solve problems such as strong hemolytic toxicity restrictions

Active Publication Date: 2021-07-23
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sea cucumber saponins have strong inhibitory activity on the proliferation of tumor cells, but the strong hemolytic toxicity of sea cucumber saponins limits its application in vivo injection

Method used

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  • A kind of sea cucumber saponin nano-liposome for injection administration and preparation method thereof
  • A kind of sea cucumber saponin nano-liposome for injection administration and preparation method thereof
  • A kind of sea cucumber saponin nano-liposome for injection administration and preparation method thereof

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Embodiment 1

[0033] A sea cucumber saponin nanoliposome available for injection, the sea cucumber saponin nanoliposome is composed of a bilayer membrane composed of phospholipids and cholesterol and the central drug sea cucumber saponin, with an encapsulation rate of over 99% and no hemolysis toxicity.

[0034] A method for preparing the sea cucumber saponin monomer by taking Philippine sea cucumber as raw material, comprising the following steps:

[0035] (1) Sea cucumber saponin extraction: sea cucumber is dried at low temperature, pulverized by a pulverizer, extracted 3 times with 60% (v / v) ethanol aqueous solution with mechanical stirring at room temperature and degreased simultaneously, the extract is centrifuged, the combined supernatant is concentrated under reduced pressure, freeze-dried, Sea cucumber total saponin A was obtained;

[0036] (2) Separation and purification of sea cucumber saponins: the total sea cucumber saponins A in step (1) are separated and purified by HP-20 mac...

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Abstract

The invention relates to the field of drug carriers, in particular to a sea cucumber saponin nano-liposome available for injection and a preparation method thereof. The sea cucumber saponin nano liposome is composed of a bimolecular layer membrane composed of phospholipids and cholesterol and a central drug sea cucumber saponin, the encapsulation rate is over 99% and has no hemolytic toxicity. The present invention prepares sea cucumber saponin nano-liposomes by wrapping sea cucumber saponin in a lipid bilayer, overcomes the hemolytic toxicity of sea cucumber triterpene saponin, and provides a safe and effective drug-carrying agent for the research and development of sea cucumber saponin as an antitumor drug system.

Description

technical field [0001] The invention relates to the field of drug carriers, in particular to a sea cucumber saponin nano-liposome available for injection and a preparation method thereof. Background technique [0002] Sea cucumber (seacucumber) belongs to Echinoermata, Holothuroidea, and Aspidochirota, and is a precious nourishing medicinal material. It is recorded in the "Food Materia Medica" of the Ming Dynasty that sea cucumbers have the health-preserving functions of replenishing vitality, nourishing internal organs and depletion. The Qing Dynasty's "Compendium of Materia Medica Supplements" listed sea cucumbers as tonic medicines. Sea cucumber contains saponins, fatty acids, gangliosides and polysaccharides and other important biologically active substances, which have a wide range of pharmacological effects such as anti-tumor, anti-coagulation, anti-virus, anti-aging and enhancing immunity. Therefore, extracting and separating biologically active substances from sea ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K31/7048A61K47/28A61K47/24A61P35/00C07J21/00
Inventor 薛长湖李红燕崔欢欢柯枭徐杰王玉明李兆杰
Owner OCEAN UNIV OF CHINA
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