Preparation method of euphausia superba active oligopeptide, euphausia superba active oligopeptide prepared by method and application

An Antarctic krill and active technology, applied in the preparation of Antarctic krill active oligopeptides, in the field of Antarctic krill active oligopeptides, can solve the problems of complex operation and high cost, and achieve simplified operation procedures, short time consumption and low sodium content Effect

Inactive Publication Date: 2019-05-17
QINGDAO CHENLAND BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Domestic patent CN 106381322 A uses Antarctic krill powder as raw material to prepare Antarctic krill active peptide, which is desalted through a nanofiltration device. Dom

Method used

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  • Preparation method of euphausia superba active oligopeptide, euphausia superba active oligopeptide prepared by method and application
  • Preparation method of euphausia superba active oligopeptide, euphausia superba active oligopeptide prepared by method and application
  • Preparation method of euphausia superba active oligopeptide, euphausia superba active oligopeptide prepared by method and application

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Effect test

Embodiment 1

[0038] (1) Raw material pretreatment: Soak and wash defatted krill powder and tap water according to the ratio of 1:15, and wash for 3 times in total. After natural sedimentation, the washing solution is removed by pouring method;

[0039](2) Enzymolysis: mix pretreated defatted krill powder (by dry weight) with distilled water at a ratio of 1:10, and add 2% alkaline protease (enzyme activity 2) from Henan Yangshao Biochemical Engineering Co., Ltd. to the mixed solution. ×10 5 u / g), hydrolysis at 60℃ for 4.5h;

[0040] (3) Enzyme inactivation: after enzymatic hydrolysis for a period of time, the temperature is raised to 90°C to inactivate the enzyme for 15 minutes;

[0041] (4) Filtration: after the enzymolysis solution is cooled, carry out vacuum filtration to obtain solid residue and filtrate. The filtrate is an Antarctic krill protein peptide solution obtained by enzymolysis from defatted krill powder, and the color is dark brown;

[0042] (5) Decolorization: add 1% activ...

Embodiment 2

[0046] (1) Raw material pretreatment: Soak and wash defatted krill powder and tap water according to the ratio of 1:15, and wash for 3 times in total. After natural sedimentation, the washing solution is removed by pouring method;

[0047] (2) Enzymolysis: pretreated defatted krill powder (by dry weight) is mixed with distilled water at a ratio of 1:10, and 2% alkaline protease (enzyme activity) of Nanning Pangbo Biological Engineering Co., Ltd. is added to the mixed solution. 2×105u / g), hydrolyzed at 60℃ for 4.5h;

[0048] (3) Enzyme inactivation: after enzymatic hydrolysis for a period of time, the temperature is raised to 90°C to inactivate the enzyme for 15 minutes;

[0049] (4) Filtration: Vacuum suction filtration is carried out after cooling the solution after the enzyme is deactivated to obtain a solid residue and a filtrate. The filtrate is an Antarctic krill protein peptide solution obtained by enzymolysis from defatted krill powder, and the color is dark brown ;

...

Embodiment 3

[0054] (1) Raw material pretreatment: Soak and wash defatted krill powder and tap water according to the ratio of 1:15, and wash for 3 times in total. After natural sedimentation, the washing solution is removed by pouring method;

[0055] (2) Enzymolysis: mix pretreated defatted krill powder (by dry weight) with distilled water at a ratio of 1:10, add 2% alkaline protease (enzyme) from Hebei Gebeida Biotechnology Co., Ltd. to the mixed solution Live 2×10 5 u / g), hydrolysis at 60℃ for 4.5h;

[0056] (3) Enzyme inactivation: after enzymatic hydrolysis for a period of time, the temperature is raised to 90°C to inactivate the enzyme for 15 minutes;

[0057] (4) Filtration: Vacuum suction filtration is carried out after cooling the solution after the enzyme is deactivated to obtain a solid residue and a filtrate. The filtrate is an Antarctic krill protein peptide solution obtained by enzymolysis from defatted krill powder, and the color is dark brown ;

[0058] (5) Decolorizati...

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Abstract

The invention discloses a preparation method of euphausia superba active oligopeptide, the euphausia superba active oligopeptide prepared by the method and application, and belongs to the field of high-value and deep processing of marine organisms. The preparation method of the euphausia superba active oligopeptide comprises the steps of pretreating a raw material of degreased euphausia powder; adding protease for enzymatic hydrolysis under suitable conditions; conducting enzyme deactivation after the enzymatic hydrolysis is completed; conducting standing, cooling and filtering to obtain a supernatant; adding activated carbon for decolorization; drying a filtered decolorized solution to obtain the euphausia superba active oligopeptide. The prepared euphausia superba active oligopeptide ishigh in homogeneity, bright and white in color, easy to dissolve in water, clear and transparent.

Description

technical field [0001] The invention belongs to the field of high-value and deep processing of marine organisms, and specifically relates to a preparation method of an Antarctic krill active oligopeptide, an Antarctic krill active oligopeptide prepared by the method and an application thereof. Background technique [0002] Antarctic krill (Euphausia superba) is a higher planktonic crustacean, biologically classified as Euphausiacea, Euphausiidae, and Euphausia. It mainly lives in shallow waters south of 50 degrees south latitude. Layer waters, body length about 4-6cm, the whole body is relatively transparent, breathes with the gills, moves by the appendages on both sides of the body, and has spherical light at the base of the eyestalk, the second to the seventh thoracic feet of the head and chest, and the abdomen It emits yellow-green light every 2-3 seconds and has a lifespan of 5-7 years. It preys on phytoplankton and zooplankton. It is currently the most abundant biologi...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14C07K1/34A61K38/01A61K8/64A61P39/06A61P9/12A61Q19/00A23L33/18A23J1/04A23J3/34
Inventor 王艳美李莉毕学一王尚龙张曾亮李八方邹圣灿
Owner QINGDAO CHENLAND BIOLOGICAL TECH CO LTD
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