Preparation method of agonist for improving stock boar sperm activity

Abstract

The invention relates to a preparation method of an agonist for improving stock boar sperm activity. The method includes the steps: (1) taking out a boar pig testicular tissue to soak the tissue; (2)cleaning the testicular tissue by PBS buffer solution; (3) adding cell culture fluid to culture the tissue, and centrifuging mixture to remove supernatant; (4) adding hyaluronidase and collagenase IVto vibrate mixture, adding cell culture fluid, and centrifuging mixture to remove supernatant; (5) adding trypsin and deoxyribonuclease to vibrate mixture, and adding cell culture fluid; (6) filter mixture by a cell screen; (7) centrifuging mixture to remove supernatant, and adding cell culture fluid; (8) performing cell culture passage; (9) culturing cell fluid, and storing the cell fluid to obtain the agonist. The agonist has the advantages that the agonist is good in application effect, low in cost and easy to produce.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of an agonist for improving boar sperm motility. Background technique [0002] The quality of boar sperm plays a vital role in boar management. The quality of sperm is directly related to the breeding conception rate and litter size of the pig farm, which in turn affects the economic benefits of the entire pig farm. Checking the semen after boar semen collection is helpful to understand the state of the boar’s sperm and grasp the reproductive performance of the boar. The routine inspection of sperm includes the amount, smell, color, vigor and density of semen. Among them, sperm motility, as an important indicator of boar sperm quality, is widely used. Sperm motility refers to the percentage of motile sperm in semen. Since only motile sperm can have normal viability and fertilization ability, sperm motility is closely related to female conception rat...

AI Technical Summary

Problems solved by technology

[0004] In the process of using the above method to improve the motility of the boar’s sperm, it was found that: (1) the intramuscular injection of testosterone belongs to the androgen class of drugs. Although the injection of testosterone can make the boar’s libido hyperactive, long-term use can cause the boar’s testis to shrink, and the sperm The production is inhibited, and side effects such as erythrocytosis, vomiting, rash, neuroangioedema, and abnormal liver function may also occur, which also have a certain impact on the boar's semen production years

(2) The main components of Yashiyong are L-carnitine, aspartic acid, VB 1 , lysine, VB 6 , glycine, etc. Although Yashiyong can effectively improve the fertilization rate of breeding livestock and poultry and reduce the mortality rate, some studies have found that: "Yashiyong" oral liquid has no obvious effect on the ejaculation volume of large white pigs and Landrace pigs, and "Yashiyong" "Imported boar health products from Italy, the price is relatively high

(3) Strengthen boar exercise, feed raw eggs, carrots and other methods every day. In actual production, it is found that the method of improving the sperm motility of breeding boars is limited and takes a long time

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