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Nitrogen-doped carbon material nano-enzyme based on nucleic acid bases or nucleic acid derivatives and preparation method and application of nano-enzyme

A nucleic acid base, nitrogen-doped carbon technology, applied in the direction of chemical analysis using catalysis, can solve the problems of difficult to achieve large-scale industrial production, complex process, high cost, etc., and achieve high-efficiency peroxidase-like catalytic activity , high nitrogen doping rate and low cost

Active Publication Date: 2019-05-24
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are many problems in the current method of preparing nitrogen-doped carbon material nanozyme, which is difficult to meet the actual application requirements.
For example, the current preparation method is complicated in process, high in cost and low in yield, and it is difficult to realize large-scale industrial production
Moreover, many methods require template sacrificial agents, and a large amount of toxic and harmful polluting wastes are produced during the preparation process.

Method used

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  • Nitrogen-doped carbon material nano-enzyme based on nucleic acid bases or nucleic acid derivatives and preparation method and application of nano-enzyme
  • Nitrogen-doped carbon material nano-enzyme based on nucleic acid bases or nucleic acid derivatives and preparation method and application of nano-enzyme
  • Nitrogen-doped carbon material nano-enzyme based on nucleic acid bases or nucleic acid derivatives and preparation method and application of nano-enzyme

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 Preparation of nitrogen-doped carbon material nanozyme based on adenine

[0028] 1. Put 5 groups of adenine powder precursors into 5 groups of alumina crucibles;

[0029] 2. Place 5 alumina crucibles with adenine powder in high-temperature tube furnaces filled with argon gas;

[0030] 3. Raise the temperature of 5 sets of high-temperature tube furnaces to 600°C, 700°C, 800°C, 900°C, and 1000°C respectively.

[0031] 4. Keep the temperature of the high-temperature tube furnace at 600°C, 700°C, 800°C, 900°C, 1000°C for carbonization, and continue the reaction for 3 hours.

[0032] 5. After carbonization, cool naturally to room temperature, take out the product, and the obtained product is adenine-based nitrogen-doped carbon material nanozyme, named ANC600, ANC700, ANC800, ANC900, ANC1000 respectively.

Embodiment 2

[0033] Embodiment 2 Preparation of nitrogen-doped carbon material nanozyme based on guanine

[0034] 1. Put 5 groups of guanine powder precursors into 5 groups of alumina crucibles;

[0035] 2. Place 5 alumina crucibles with guanine powder in a high-temperature tube furnace filled with argon gas;

[0036] 3. Raise the temperature of 5 sets of high-temperature tube furnaces to 600°C, 700°C, 800°C, 900°C, and 1000°C respectively.

[0037] 4. Keep the temperature of the high-temperature tube furnace at 600°C, 700°C, 800°C, 900°C, 1000°C for carbonization, and continue the reaction for 3 hours.

[0038]5. After carbonization, cool down to room temperature naturally, and take out the product. The obtained product is a carbon material nanozyme based on guanine nitrogen doping, named GNC 600, GNC700, GNC800, GNC900, and GNC1000 respectively.

Embodiment 3

[0039] Example 3 Preparation of nitrogen-doped carbon material nanozyme based on cytosine

[0040] 1. Put cytosine powder precursors in 5 groups of alumina crucibles respectively;

[0041] 2. Put 5 alumina crucibles with cytosine powder in a high-temperature tube furnace filled with argon gas;

[0042] 3. Raise the temperature of 5 sets of high-temperature tube furnaces to 600°C, 700°C, 800°C, 900°C, and 1000°C respectively.

[0043] 4. Keep the temperature of the high-temperature tube furnace at 600°C, 700°C, 800°C, 900°C, 1000°C for carbonization, and continue the reaction for 3 hours.

[0044] 5. After carbonization, cool naturally to room temperature, take out the product, and the obtained product is cytosine-based nitrogen-doped carbon material nanozyme, named CNC600, CNC700, CNC800, CNC900, CNC1000 respectively.

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Abstract

The invention discloses a nitrogen-doped carbon material nano-enzyme based on nucleic acid bases or nucleic acid derivatives. Particularly, the nitrogen-doped carbon material nano-enzyme refers to thecarbon material nano-enzyme prepared by carbonizing nucleic acid bases or nucleic acid derivatives in an inert gas environment at different temperature. The invention further discloses a preparationmethod and application of the nitrogen-doped carbon material nano-enzyme based on nucleic acid bases or nucleic acid derivatives. The preparation method has the advantages of being environmentally friendly, economical, low in cost, simple in processing, high in nitrogen doping ratio, capable of realizing production in batch, and the like. The nitrogen-doped carbon material nano-enzyme prepared bythe method has high quasi-peroxide enzyme catalytic activity.

Description

technical field [0001] The invention belongs to the technical field of heterogeneous element-doped carbon nanomaterials, and specifically relates to nitrogen-doped carbon material nanozymes based on nucleic acid bases or derivatives thereof, as well as their preparation methods and applications. Background technique [0002] Biological enzymes are a type of biocatalysts that life depends on, and can efficiently catalyze a series of chemical reactions under extremely mild physiological conditions. Biological enzymes are mostly proteins, and there are also a few nucleic acids, which have exquisite three-dimensional structures, which endow them with extremely high catalytic activity and substrate selectivity. However, biological enzymes are difficult to extract, separate and purify, have high production costs, poor stability and are easily inactivated, and are sensitive to environmental conditions. These shortcomings greatly limit the large-scale production and application of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/10
Inventor 魏辉林世超
Owner NANJING UNIV
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