Ferric oxide nano-cluster mimic enzyme, preparation method and method for rapid detection of lactic acid

A technology of ferric tetroxide and nanoclusters, which is applied in the fields of nanotechnology and analysis and detection, can solve the problems of expensive equipment, low specificity, and easy interference, and achieves excellent peroxidase-like activity and high simulated peroxidation. The effect of enzyme catalytic ability

Active Publication Date: 2016-06-29
兰州创力测控技术有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Some of these methods have the problems of low specificity, susceptibility to interference, and low accuracy, while others require expensive equipment and require high requirements for operators. A new method for detecting lactic acid with high selectivity and high sensitivity will greatly promote the detection level of lactic acid in clinical medicine

Method used

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  • Ferric oxide nano-cluster mimic enzyme, preparation method and method for rapid detection of lactic acid
  • Ferric oxide nano-cluster mimic enzyme, preparation method and method for rapid detection of lactic acid
  • Ferric oxide nano-cluster mimic enzyme, preparation method and method for rapid detection of lactic acid

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Experimental program
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Effect test

Embodiment 1- 4

[0040] Preparation of embodiment 1-ferric oxide nanocluster mimetic enzyme:

[0041] 1) Dissolve 2.5mmol ferric chloride and 15mmol anhydrous sodium acetate in 40mL ethylene glycol, then add 0.54mL deionized water and stir to form a uniformly dispersed solution;

[0042] 2) Put the solution obtained in step 1 into a hydrothermal kettle, put it into an oven heated to 180°C to react for 12 hours, and then cool to room temperature with the furnace;

[0043]3) Pour out the supernatant in the reaction kettle, take out the precipitated product, wash with ethanol and deionized water three times respectively, and dry in a vacuum oven at 60°C for 10 hours to obtain a solid sample that is ferric oxide nanoclusters .

[0044] The morphology, structure and magnetic properties of the obtained Fe3O4 nanoclusters were characterized as follows: Figure 1-3 Shown:

[0045] From figure 1 (A) Fe3O4 nanoclusters are observed in transmission electron microscope composed of many small Fe 3 o ...

Embodiment 2-6

[0048] Examples 2-6, preparation of iron ferric oxide nanocluster mimetic enzymes: the steps are the same as in Example 1, and the specific amounts of raw materials and reaction conditions are shown in Table 1.

[0049]

Embodiment 2

[0050] The morphological characterization of the iron ferric oxide nanoclusters obtained under the specific reaction conditions of embodiment 2 and embodiment 4 are respectively as follows Figure 4 (A) and Figure 4 (B), where Figure 4 The insets in (A) and (B) are selected high-magnification TEM images of a Fe3O4 nanocluster.

[0051] From Figure 4 It can be seen that the average particle size of the iron ferric oxide nanoclusters obtained under the specific reaction conditions of embodiment 2 and embodiment 4 is about 40nm and 130nm respectively, and these two kinds of ferric oxide nanoclusters are still composed of many very small Fe 3 o 4 Cluster-like loose particles formed by self-assembly of nanocrystals.

[0052] "Characterization of Ferric Oxide Nanoclusters Mimicking Peroxidase Properties"

[0053] Mix 100 μL of 1 mg / mL ferric oxide nanocluster simulated enzyme stock solution, 100 μL of 100 μM H 2 o 2 Aqueous solution and 100 μl ethanol solution of 3,3',5,5...

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Abstract

The invention discloses a ferroferric oxide nanocluster mimetic enzyme, a preparation method and a method for rapid detection of lactic acid with the ferroferric oxide nanocluster mimetic enzyme. The mimetic enzyme is a cluster-shaped loose particle formed by self-assembly of fine ferroferric oxide nanocrystals, and has a large specific surface area. The preparation method of the mimetic enzyme includes the steps of: dissolving ferric trichloride and anhydrous sodium acetate in ethylene glycol, then adding deionized water and performing stirring to form a uniformly dispersed solution; loading the solution obtained in step A into a hydrothermal reaction kettle, and putting the kettle into an oven heated to 160DEG C-200DEG C to react; and carrying out washing and vacuum drying, thus obtaining a solid sample. The invention not only provides an effective mimetic peroxidase material, but also establishes a new high selectivity method for simple and sensitive detection of lactic acid, and the method can be widely applied to clinical medical diagnosis and food safety monitoring.

Description

technical field [0001] The invention belongs to the technical field of nanotechnology and analysis and detection, and in particular relates to a ferric oxide nano-cluster imitation enzyme, a preparation method and a method for rapidly detecting lactic acid by using the same. Background technique [0002] Since the discovery of the enzyme-mimicking properties of nanomaterials, nanomaterial-mimicking enzymes have been widely used in immunomedical detection, glucose and hydrogen peroxide detection, and removal of organic pollutants due to their advantages such as simple preparation and purification, easy storage, low price, and stable performance. and many other fields. The catalytic activity of nanomaterials imitating enzymes has a great relationship with its specific surface area. The larger the specific surface area, the more catalytic active sites on the surface of the material, and the higher the catalytic activity of the material similar to natural enzymes. The surface a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31C01G49/06G01N21/75B82Y30/00B82Y40/00
Inventor 左显维韩根亮张彪冯治棋宋玉哲郑礴
Owner 兰州创力测控技术有限公司
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