Microorganism concentration and purification device and microorganism concentration and purification method based on device

A purification method and microbial technology, applied in the preparation of test samples, etc., can solve the problems of difficult continuous on-line processing, low recovery rate of microbial concentration technology, high cost of microbial purification technology, etc., achieve continuous concentration and purification, reduce purification costs, The effect of avoiding the loss of microbial adhesion

Inactive Publication Date: 2019-05-28
中山柏生科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a microbial concentration and purification device to solve the problems of low recovery ra...

Method used

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  • Microorganism concentration and purification device and microorganism concentration and purification method based on device
  • Microorganism concentration and purification device and microorganism concentration and purification method based on device
  • Microorganism concentration and purification device and microorganism concentration and purification method based on device

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Embodiment

[0034] Example: see attached figure 1 to attach Figure 5 , the present embodiment provides a microorganism concentration and purification device, including an acoustofluidic device 1 and an ultrasonic wave generating unit 15 disposed on the acoustofluidic device 1, the acoustofluidic device 1 is mainly used for concentrating and purifying microorganisms; The acoustofluidic device 1 is provided with a microfluidic chamber 11, a sample inlet 12 and a sample outlet 13, and the sample inlet 12 and the sample outlet 13 communicate with the microfluidic chamber 11 to form a microbial fluid sample that can The circulating environment; the microfluidic chamber 11 is used as the main place for concentration and purification, so that microorganisms with different characteristics are subjected to different acoustic radiation forces generated by the ultrasonic sounding unit, and gather in the microfluidic chamber under the action of this force Different space heights are convenient for ...

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Abstract

The invention discloses a microorganism concentration and purification device. The device comprises an acoustic fluid device and an ultrasonic generation unit arranged on the acoustic fluid device; the acoustic fluid device is provided with a microfluidic cavity, a sample inlet and sample outlets, the sample outlets at least comprise the first sample output and the second sample outlet which are arranged in the up-down direction, and the sample inlet and the sample outlets are communicated with the microfluidic cavity; the ultrasonic generation unit is located between the sample inlet and thesample outlets and arranged at the upper side or the lower side of the microfluidic cavity for upwards generating an ultrasonic wave, the ultrasonic wave is a standing wave, through acoustic wave radiation force generated by the standing wave, microorganism samples which flow through the microfluidic cavity form layered microorganism streams, and different layers of microorganism samples flow outalong different sample outlets respectively. Through the ultrasonic standing wave generated by the ultrasonic generation unit, microorganisms are subjected to acoustic wave radiation force and purified, adhesion loss of the microorganisms is avoided, the recovery rate is increased, the samples are concentrated, and the cost is lowered.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganism concentration and purification, in particular to a microorganism concentration and purification device and a microorganism concentration and purification method based thereon. 【Background technique】 [0002] In real life, the microbial content in drinking water is relatively low compared to medical microbiological testing samples. For example, the standard detection limit of parasites (flagellates, cryptosporidium) is 1 / 10 liters of water; the standard detection limit of Escherichia coli is 1 CFU / 100ml of water. In order to make the detection limit of microorganisms in drinking water reach the standard detection limit, the concentration and purification of microorganisms in drinking water samples is a key step in the process of microbial detection in drinking water. [0003] The existing drinking water microbial concentration adopts the method of filtration membrane filtration and high-p...

Claims

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Application Information

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IPC IPC(8): G01N1/40G01N1/34
Inventor 雷磊黄伟张鑫
Owner 中山柏生科技有限公司
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