The invention discloses a microorganism concentration and purification device. The device comprises an acoustic fluid device and an ultrasonic generation unit arranged on the acoustic fluid device; the acoustic fluid device is provided with a microfluidic cavity, a sample inlet and sample outlets, the sample outlets at least comprise the first sample output and the second sample outlet which are arranged in the up-down direction, and the sample inlet and the sample outlets are communicated with the microfluidic cavity; the ultrasonic generation unit is located between the sample inlet and thesample outlets and arranged at the upper side or the lower side of the microfluidic cavity for upwards generating an ultrasonic wave, the ultrasonic wave is a standing wave, through acoustic wave radiation force generated by the standing wave, microorganism samples which flow through the microfluidic cavity form layered microorganism streams, and different layers of microorganism samples flow outalong different sample outlets respectively. Through the ultrasonic standing wave generated by the ultrasonic generation unit, microorganisms are subjected to acoustic wave radiation force and purified, adhesion loss of the microorganisms is avoided, the recovery rate is increased, the samples are concentrated, and the cost is lowered.