Bovine brucellosis and bovine tuberculosis bivalent test strip detection card
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A technology of Brucella bovis and detection card is applied in the field of animal disease detection products, which can solve the problems of long time, complicated operation of intradermal allergic reaction, and difficulty in meeting a large number of quarantine of bovine tuberculosis.
Inactive Publication Date: 2019-05-28
CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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Problems solved by technology
Among them, the operation of intradermal allergy is cumbersome and time-consuming, and it is difficult to meet the needs of a large number of bovine tuberculosis quarantines
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Embodiment 1
[0011] Embodiment 1: Preparation of bovine brucellosis antibody and bovine tuberculosis gamma-interferon colloidal gold test strip detection card
[0012] 1. Required instruments and reagents
[0013] 1 XYZ-3000 three-dimensional spraying device: product of American Biodot Company.
[0014] 2 Gold standard consumables: test card, glass fiber, absorbent cotton, support pulley, products of Shanghai Gold Standard Company.
[0015] 3 Chloroauric acid: provided by Shanghai Chemical Reagent Company, and used as a 1% solution with deionized water.
[0016] 4 Monoclonal antibodies: Mabtak MT17.1 and MT307 monoclonal antibodies.
[0017] 5 Brucella lipopolysaccharide antigen: prepared by the laboratory itself.
[0018] 6 Rabbit anti-bovine antibody: product of SIGMA Company.
[0019] 7 Goat anti-mouse antibody: product of SIGMA Company.
[0020] 8 nitrocellulose membrane (NC) membrane: FF170, the product of Millipore Company,
[0021] 9 Cutting machine: product of American Biodot...
Embodiment 2
[0046] Embodiment 2: the use method of bovine brucellosis antibody and bovine tuberculosis gamma-interferon colloidal gold test strip detection card
[0047] 1. How to use:
[0048] (1) Preparation method of stimulation supernatant
[0049] 1 Blood collection, collect blood from cattle (at least 2mL) into a heparin anticoagulant tube, gently invert several times to mix the blood to dissolve the heparin. Delivered to the laboratory at room temperature (22±5°C, avoid overheating or undercold temperature) and cultured within 8 hours after blood collection.
[0050] 2 Add sample, add anticoagulant blood to 24-well tissue culture plate, 1.5mL anticoagulant blood per well.
[0051] 3. Add stimulating antigen, aseptically add 100 μL bovine PPD to each well.
[0052] 4 Incubate, incubate the tissue culture plate containing blood and antigen in a humidified incubator at 37°C for 16-24 hours.
[0053] 5 Collect the sample, carefully draw about 400 μL of the upper layer of plasma wit...
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Abstract
The invention provides a colloidal gold test strip detection card capable of simultaneously detecting a bovine brucellosis antibody and bovine tuberculosis gamma-interferon. The colloidal gold test strip detection card comprises a carrier plate, a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad and the water absorption pad are located at the two ends of the carrier plate; the nitrocellulose membrane is located in the middle of the carrier plate; the gold label pad loaded with a gold-labeled anti-gamma-interferon monoclonal antibody anda gold-labeled rabbit anti-bovine secondary antibody is arranged on the junction of the sample pad and the nitrocellulose membrane; the edge of one end of the gold label pad is overlapped under the sample pad, and the edge of the other end of the gold label pad is overlapped on the nitrocellulose membrane; a bovine tuberculosis detection line, a brucellosis antibody detection line and a quality control line are arranged from the side, close to the gold label pad, of the nitrocellulose membrane; the bovine tuberculosis detection line is coated with an anti-gamma-interferon monoclonal antibodyMT17.1, the brucellosis antibody detection line is coated with brucella lipopolysaccharide antigens, and the quality control line C is coated with goat anti-mouse IgG. The detection card can be used for effectively detecting bovine brucellosis and bovine tuberculosis.
Description
technical field [0001] The invention belongs to the technical field of animal epidemic detection products, in particular to a bovine brucellosis and bovine tuberculosis dual test strip detection card. Background technique [0002] Brucellosis is a zoonotic disease that not only harms cattle, sheep, pigs and other animals, causing serious economic losses, but also transmits the disease to humans through infected animals or livestock products, causing fever, Orchitis, miscarriage and arthritis and other diseases, severe cases even loss of labor and fertility. In recent years, the prevalence of human brucellosis has been serious in my country, and the only source of infection of human brucellosis is animal brucellosis. The main method to control animal brucellosis is to detect positive animals and eliminate them in time. Bovine brucellosis is an animal disease that must be notified by the International Organization for Animal Health (OIE). It is a second-class animal infectio...
Claims
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Application Information
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