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Full-automatic molecular diagnosis system and molecular diagnosis method

An automatic molecular and diagnostic system technology, applied in biochemical equipment and methods, biochemical instruments, enzymology/microbiology devices, etc., can solve problems such as inflexibility, low throughput, and inability to meet application requirements, and reduce work intensity , good cost control, and the effect of reducing cross-contamination rate

Pending Publication Date: 2019-05-31
SUZHOU SYM BIO LIFESCI CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the above-mentioned 1) batch processing mode is used. This mode has a large sample processing throughput, but it is not flexible. A batch of samples must be collected before starting to work. For example, after the instrument has started to work, no additional samples can be added for experiments; the adopted The above 2) single-sample processing mode, this mode is flexible in operation, but the throughput is too small, when the sample size is large, it cannot meet the application requirements, and requires too much manual participation

Method used

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  • Full-automatic molecular diagnosis system and molecular diagnosis method
  • Full-automatic molecular diagnosis system and molecular diagnosis method
  • Full-automatic molecular diagnosis system and molecular diagnosis method

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Embodiment 1

[0042] Such as Figure 1-2 As shown, the present embodiment provides a fully automatic molecular diagnostic system, including: at least one nucleic acid extraction reagent strip, each hole in the nucleic acid extraction reagent strip is pre-packaged with reagents such as extraction solution and washing solution required for nucleic acid extraction; At least one nucleic acid extraction module 103 for nucleic acid extraction of the nucleic acid extraction reagent strip after filling the sample, the nucleic acid extraction reagent strip flows to the nucleic acid extraction module 103 through the extraction reagent strip transmission channel 102; at least one is used to receive the nucleic acid extraction module 103 The nucleic acid amplification reagent strip of the extracted nucleic acid; and at least one amplification reading module 107 for amplifying and reading the nucleic acid amplification reagent strip filled with nucleic acid, the nucleic acid amplification reagent strip p...

Embodiment 2

[0050] This embodiment provides a fully automatic molecular diagnosis method for non-disease diagnosis and non-treatment purposes, as shown in Figure 3-4, which specifically includes the following steps:

[0051] Step 1, the nucleic acid extraction reagent strip is transferred from the nucleic acid extraction reagent strip storage bin 101 to the extraction reagent strip transmission channel 102;

[0052] Step 2, the sample loading arm 109 injects the sample from the test tube in the sample platform to the nucleic acid extraction reagent strip;

[0053] Step 3, the nucleic acid extraction reagent strip is transferred from the nucleic acid extraction reagent strip transmission channel 102 to the nucleic acid extraction module 103 for nucleic acid extraction; the system is equipped with several nucleic acid extraction modules 103, and the number of its configuration can be based on the design requirements of the instrument throughput Customization is required. For example, it tak...

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Abstract

The invention discloses a full-automatic molecular diagnosis system and a diagnosis method. The diagnosis system comprises at least one nucleic acid extraction reagent strip, at least one nucleic acidextraction module used for carrying out nucleic acid extraction on the nucleic acid extraction reagent strip filled with a sample, at least one nucleic acid amplification reagent strip for receivingnucleic acid extracted by the nucleic acid extraction module and at least one amplification reading module used for amplifying and reading the nucleic acid amplification reagent strip filled with thenucleic acid. According to the full-automatic molecular diagnosis system and the full-automatic molecular diagnosis method, molecular diagnosis is carried out in a working mode of continuous serial processing of a single sample, samples can be processed in batch, a sporadic single sample can be processed, a new sample can also be processed at any time for experiment, various requirements of customers can be met more flexibly, and the working intensity of operators is reduced.

Description

technical field [0001] The invention belongs to the field of life medical detection and diagnosis, and in particular relates to a fully automatic molecular diagnosis system and a molecular diagnosis method. Background technique [0002] The existing fully automatic molecular diagnosis system has two modes: 1) batch processing mode: such as the fully automatic nucleic acid detection reaction system produced by the Swiss Hamilton Company, which mainly includes two parts: a reagent distribution mechanism and a nucleic acid extraction mechanism, wherein the reagent distribution mechanism has A sample loading platform, on which deep well plates (96 wells, 48 ​​wells, etc.) In the well plate, the deep well plate after adding the sample is transferred to the nucleic acid extraction mechanism by the robot arm of the system for extraction; 2) Single sample processing mode, such as molecular diagnosis POCT products. [0003] However, the above-mentioned 1) batch processing mode is us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/34
Inventor 许海峰许明邓永锦
Owner SUZHOU SYM BIO LIFESCI CO LTD
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