Slice manufacturing device and method used for microscopic observation of filamentous fungi morphological characteristic

Abstract

The invention belongs to the mycology research field and discloses a slice manufacturing device and method used for microscopic observation of a filamentous fungi morphological characteristic. The slice manufacturing device used for the microscopic observation of the filamentous fungi morphological characteristic comprises a glass culture dish. Circular filter paper is placed in the glass culturedish. A single concave glass slide is placed on the surface of the circular filter paper. The central section of the single concave glass slide is provided with a groove. A sterile cover glass slice is placed on the upper portion of the groove. The structure is simple, a design is scientific, operation is simple, labor saving and time saving are achieved, and the device and the method can be directly used for microscopically observing the morphological characteristic of filamentous fungi under a natural growth state. Hypha overlap, spore shedding, bubble generation and damages to the natural growth state of thalluses during a traditional slice manufacturing process are avoided, and dynamic development processes of a spore production mode, a spore production structure and spore formation offungi can be clarified. The accuracy and the reliability of species identification of the filamentous fungi are ensured.

Description

technical field [0001] The invention belongs to the field of mycological research, and in particular relates to a film-making device and method for microscopic observation of morphological characteristics of filamentous fungi. Background technique [0002] For a long time, the research on the classification of filamentous fungi has been mainly based on the description and identification of the morphological characteristics of the fungi. The sporulation mode, sporulation structure and spore morphology of the fungi are the main criteria for the definition of the genus and species. Biological slides are very important. [0003] At present, the microscopic observation of the morphological characteristics of filamentous fungi commonly used in the industry is mainly the mycelia picking method: first drop a drop of floating agent in the center of the slide, and then pick a small amount from the culture medium with a fine needle. The filamentous fungal culture suitable for cultivat...

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Problems solved by technology

[0005] (1) Frequent slide preparation is required for each observation, and it is difficult to obtain biological slides with good observation effect, which requires a large number of slides, which is time-consuming and labor-intensive;

[0006] (2) Mycelia picking can easily cause mycelium to stagger and overlap, making it difficult to prepare the best biological slides, which affects the microscopic observation of the typical morphological characteristics of fungi;

[0007] (3) During the process of picking mycelium and placing it in the floating carrier and adding a cover glass, it is very easy to induce the floating carrier to generate bubbles, which affects the microscopic observation effect of the fungal morphological characteristics;

[0008] (4) Picking mycelium and adding a cover glass can easily destroy the natural growth state of mycelia, causing fungal spores to fall off, making it difficult for the prepared biological slides to truly reflect the sporulation mode of fungi and the dynamic development process of spore formation. Affect the accuracy and reliability of filamentous fungal species identification

[0009] To sum up, the existing method of making slices can easily destroy the natural growth state of the fungus, causing a series of problems such as hyphae overlapping, spore shedding and air bubble generation, which interferes with the microscopic observation of the morphological characteristics of filamentous fungi and affects the quality of the filamentous fungi. Morphological identification

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