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Method for simultaneous determination of multiple components in traditional Chinese medicine preparations based on lc-ms technology

A technology of LC-MS and determination method, which is applied in the direction of measuring devices, material separation, and analysis of materials, etc., can solve the problems of single component quality control method, lack of representativeness and comprehensiveness, and few basic researches on Tianlong Tongluo Capsules. Achieve the effect of improving quality control level, easy operation, feasibility and advanced

Active Publication Date: 2022-02-18
MUDANJIANG YOUBO PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are relatively few researches on the material basis of Tianlong Tongluo Capsules, and the content determination of the current standard only measures gastrodin, the ingredient in Gastrodia elata. The quality control method of ingredients is relatively single and lacks better representativeness and comprehensive

Method used

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  • Method for simultaneous determination of multiple components in traditional Chinese medicine preparations based on lc-ms technology
  • Method for simultaneous determination of multiple components in traditional Chinese medicine preparations based on lc-ms technology
  • Method for simultaneous determination of multiple components in traditional Chinese medicine preparations based on lc-ms technology

Examples

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Effect test

Embodiment 1

[0033] Example 1 Establishment of a method for simultaneously determining the content of multiple components in Tianlong Tongluo Capsules based on LC-MS technology

[0034] 1. Extract Tianlong Tongluo Capsules

[0035] Ultrasonic extraction method is adopted as the sample extraction method of Tianlong Tongluo Capsules, and the quantity of compounds measured by HPLC-MS and the content of main compounds are used as the investigation index, and the extraction solvent type (methanol, ethanol, water, etc.) , 50% methanol-water and 50% ethanol-water), the composition of the extraction solvent (10%, 30%, 50%, 70% and 90% ethanol-water), the ratio of solid to liquid (1:5, 1:20 , 1:80, 1:160, 1:320, 1:640, 1:1280, 1:2560), extraction temperature (20°C, 30°C, 40°C, 50°C), extraction time (30min, 60min, 90min , 120min) and so on. The optimal extraction conditions for Tianlong Tongluo capsules are 50% ethanol-water ultrasonic extraction for 30 minutes, the extraction temperature is 20°C...

Embodiment 2

[0064] Simultaneous Determination of 31 Kinds of Component Contents in Example 2 Tianlong Tongluo Capsule Sample

[0065] According to the method established in Example 1, 10 batches of Tianlong Tongluo Capsules were assayed. Prepare 10 batches of the test solution of Tianlong Tongluo Capsules. The preparation method is: accurately weigh 0.1g of the sample, add 32mL of 50% ethanol, weigh it with a bottle, extract it by ultrasonic extraction at 20°C for 30min at 100Hz, and obtain the extract after filtration , the extract was supplemented with 50% ethanol for weight loss, after mixing, centrifuged at 13000rpm for 10min, got the supernatant, added an appropriate amount of internal standard (petgenin, the final concentration was 83.33 μg / mL; glycyrrhetinic acid, the final concentration was 83.33 μg / mL ) as the test solution, inject 5 μL to detect, record the ratio (Pa) of the peak area of ​​the measured component to the peak area of ​​the internal standard compound, and substitut...

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Abstract

The invention relates to a method for simultaneously measuring the contents of various components of a Chinese medicine preparation. Specifically, liquid chromatography-mass spectrometry (LC-MS) technology is used in combination with multiple reaction monitoring (MRM) to establish a variety of ingredients in Tianlong Tongluo Capsules. Simultaneous determination of ingredient content. The method can quickly and effectively determine the content of 31 ingredients in Tianlong Tongluo Capsules at the same time. The method is accurate, sensitive, specific and reproducible. product quality.

Description

technical field [0001] The invention belongs to the technical field of quality control of traditional Chinese medicines, and relates to a method for simultaneously determining the contents of various components in traditional Chinese medicine preparations based on liquid chromatography-mass spectrometry (LC-MS) technology. Specifically, a liquid chromatography-mass spectrometry technique combined with multiple reaction monitoring (MRM) was used to establish a simultaneous determination method for the contents of multiple components in Tianlong Tongluo Capsules to improve its quality control level. Background technique [0002] Tianlong Tongluo Capsule is composed of five traditional Chinese medicines such as earthworm, gastrodia elata, salvia miltiorrhiza, Chuanxiong, and artificial bezoar, according to the classic Chinese medicine compatibility theory of "junchenzuoshi", which has the effects of dispelling wind and dredging collaterals, promoting blood circulation and removi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/04
Inventor 李振国陈艳明郑顺亮王思瑶李萍杨华
Owner MUDANJIANG YOUBO PHARMA CO LTD