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Preparation method and application of hyacinth bletilla polyphenols

The technology of Bletilla striata polyphenols and Bletilla striata is applied in the field of preparation of Bletilla striata polyphenols, can solve the problems of less research on fibrous roots and the like, and achieve the effects of short extraction time, production cost saving and high preparation purity

Active Publication Date: 2019-06-11
黄山峰源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scholars at home and abroad have mostly focused on the tubers of Bletilla striata, and relatively few studies on fibrous roots.

Method used

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  • Preparation method and application of hyacinth bletilla polyphenols
  • Preparation method and application of hyacinth bletilla polyphenols
  • Preparation method and application of hyacinth bletilla polyphenols

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 A kind of preparation of bletilla striata polyphenol

[0027] A preparation of bletilla striata polyphenols, comprising the steps of:

[0028] (1) Raw material pretreatment: wash and dry the bletilla striata fibrous root raw material and cut into uniform and thin bletilla striata fibrous root raw material, and carry out high-pressure moist heat sterilization;

[0029] (2) Microbial solid fermentation: Inoculate the uniform and thin Bletilla striata fibrous root raw material into the versicolor versicolor bacterium suspension of 0.05% respectively, and the concentration of the bacterium suspension is 1×10 6 CFU / mL, the temperature of the fermentation culture is 35°C, and the time of the fermentation culture is 3 days. Freeze-drying the fibrous root of Bletilla striata after solid fermentation, crushing and passing through a 100-mesh sieve to obtain the fermented product powder of fibrous root of Bletilla striata.

[0030] (3) Composition of the two-phase a...

Embodiment 2

[0034] Embodiment 2 A kind of preparation of bletilla striata polyphenol

[0035] A preparation of bletilla striata polyphenols, comprising the steps of:

[0036] (1) Raw material pretreatment: wash and dry the bletilla striata fibrous root raw material and cut into uniform and thin bletilla striata fibrous root raw material, and carry out high-pressure moist heat sterilization;

[0037] (2) Microbial solid fermentation: Inoculate the uniform and thin Bletilla striata fibrous root raw material respectively with 5% Schizophyllum and Saccharomyces cerevisiae (1:1) microbial suspensions with a concentration of 1×10 8 CFU / mL, the temperature of the fermentation culture is 28°C, and the time of the fermentation culture is 8 days. Freeze-drying the fibrous root of Bletilla striata after solid fermentation, crushing and passing through a 100-mesh sieve to obtain the fermented product powder of fibrous root of Bletilla striata.

[0038] (3) Two-phase aqueous system composition: Prep...

Embodiment 3

[0042] Embodiment 3 A kind of preparation of bletilla striata polyphenol

[0043] A preparation of bletilla striata polyphenols, comprising the steps of:

[0044] (1) Raw material pretreatment: wash and dry the bletilla striata fibrous root raw material and cut into uniform and thin bletilla striata fibrous root raw material, and carry out high-pressure moist heat sterilization;

[0045] (2) Microbial solid fermentation: Inoculate the Bletilla striata fibrous root raw material of described even fine shape respectively and be 3% Bifidobacterium bifidum microorganism bacterium suspension, bacterium suspension concentration is 1 * 10 8 CFU / mL, the temperature of the fermentation culture is 35°C, and the time of the fermentation culture is 4 days. Freeze-drying the fibrous root of Bletilla striata after solid fermentation, crushing and passing through an 80-mesh sieve to obtain a fermented product powder of fibrous root of Bletilla striata.

[0046] (3) Composition of the two-ph...

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Abstract

The invention discloses a preparation method of hyacinth bletilla polyphenols. According to the method, release of soluble polyphenols in the hyacinth bletilla fibrous roots is promoted through microbial solid state fermentation. The method comprises the following steps: preparing a hyacinth bletilla polyphenol component by adopting an ultrasonic-negative pressure cavitation coupled aqueous two-phase extraction technology, removing biomacromolecules such as polysaccharide and protein in the hyacinth bletilla polyphenol component by utilizing an ultrafiltration membrane interception technology,and conducting enriching and purifying by combining macroporous adsorption resin to obtain the hyacinth bletilla polyphenols with high purity and strong activity. Experiments show that the antibacterial, hemostatic and anti-inflammatory effects of the hyacinth bletilla polyphenols are discovered; and meanwhile, Chinese herbal medicine toothpaste containing the hyacinth bletilla polyphenols is developed.

Description

technical field [0001] The invention relates to a preparation method and application of bletilla striata polyphenols, belonging to the field of biotechnology. Background technique [0002] Bletilla striata, also known as white root, purple orchid, ground screw, etc., is the dry tuber of the orchid plant Bletilla striata, and is one of the traditional Chinese medicinal materials commonly used in my country. Bletilla striata has high medicinal value. It has the effects of nourishing the lungs, astringent hemostasis, detumescence, and muscle growth. It can be used to treat symptoms such as lung injury and hemoptysis, sore swelling, surgical trauma, stomach and peptic ulcer pain. The main chemical components of Bletilla striata are bibenzyls, phenanthrenes and their derivatives, polysaccharides, flavonoids, etc., which have anti-tumor, anti-virus, anti-oxidation, anti-inflammation, inhibition of tyrosinase and promotion of wound healing. At present, the "Chinese Pharmacopoeia" ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9794A61K8/92A61K8/9789A61K8/49A61Q11/00
Inventor 吴永祥章鏴吴丽萍王卫东耿玉闯卢玮玮戴毅胡长玉方建新吕顺清
Owner 黄山峰源生物科技有限公司
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