Spider silk protein bone nails and preparation method thereof

A spider silk protein and spider silk technology, applied in the field of biomedicine, can solve the problems of low breaking strength, easy fatigue, no bone conduction, etc., and achieve good compatibility and affinity

Active Publication Date: 2019-06-11
江西丝科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degradation of materials such as polylactic acid (PLA) will form some acidic products, which will have a negative impact on human tissues.
These materials cause acidic buildup as they degrade and are not osteoconductive, thus failing to promote bone cell growth
This type of material degrades quickly and is not suitable for the repair of main load-bearing bones
In addition, the mechanical properties of such materials cannot fully meet the clinical needs of bone repair, and it is necessary to improve their mechanical properties by adding reinforcing fibers.
[0004] At the same time, PLA toughened ceramic-based materials are also used to prepare bone implants, which can achieve the same mechanical properties as the bone nail and increase the biocompatibility, but the bone nail cannot be completely degraded.
[0005] In addition, although bone screws made of calcium phosphate bone materials have degradability, good osteoconductivity, and biocompatibility, and calcium phosphate bone screws can bear compressive loads, they have low fracture strength, brittleness, and fatigue

Method used

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  • Spider silk protein bone nails and preparation method thereof
  • Spider silk protein bone nails and preparation method thereof
  • Spider silk protein bone nails and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 of the present invention provides a method for preparing a spider silk protein bone nail, such as figure 1 As shown, the preparation method of the spidroin protein bone nail comprises the following steps.

[0039] Step 1, preparing a spider silk protein solution.

[0040] The spider silk protein solution may be a solution of natural spider silk protein, or a solution of gene recombinant spider silk protein. Next, gene recombinant spider silk protein is introduced as an example. Firstly, the engineered bacteria expressing gene recombinant spider silk protein through high-density fermentation were dissolved in the first Tris-HCl (PH=8) / NaCl / Imidazole solution (Tris-HCl concentration 20mM, NaCl concentration 150mM, Imidazole range 5mM), Destroy the bacteria using a bacteriostasis instrument. Centrifuge the broken bacteria to collect the supernatant; then incubate the supernatant in a constant temperature water bath at 80°C for 24 hours, centrifuge to collec...

Embodiment 2

[0056] In step 1, the first Tris-HCl (PH=8) / NaCl / Imidazole solution is the solution of Tris-HCl concentration 5mM, NaCl concentration 200mM, Imidazole range 10mM); The second Tris-HCl (PH=8) / NaCl / Imidazole solution is Tris-HCl concentration 5mM, NaCl concentration 200mM, Imidazole range 40mM solution; the third Tris-HCl(PH=8) / NaCl / Imidazole solution is Tris-HCl concentration 50mM, NaCl concentration 200mM, Imidazole range 300mM solution . Other operations are the same as in Example 1.

[0057] The spider silk protein bone nail prepared by the method for preparing the spider silk protein bone nail provided in Example 2 of the present invention has good affinity with the human body, no immune rejection, good compatibility with human tissue, and no toxic effect on the human body; In the preparation method of the spider silk protein bone nail provided by the embodiment of the invention, by adding a functional drug in the dissolution stage of the spider silk protein, or soaking t...

Embodiment 3

[0062] In step 1, the natural spider silk protein is dissolved in water, frozen at -40°C, and vacuum-frozen in a vacuum freeze dryer for 250 hours, the vacuum pressure is 0.001mBar, and the temperature is -80°C; anhydrous natural spider silk is obtained protein, and then 1 weight part of anhydrous natural spider silk protein was dissolved in 0.5 weight part of hexafluoroisopropanol, and the dissolution temperature was 27.5 degrees Celsius to obtain the spider silk protein solution. Other steps are identical with embodiment 1.

[0063]The spider silk protein bone nail prepared by the preparation method of the spider silk protein bone nail provided in Example 3 of the present invention has good affinity with the human body, no immune rejection, good compatibility with human tissue, and no toxic effect on the human body; In the preparation method of the spider silk protein bone nail provided by the embodiment of the invention, by adding a functional drug in the dissolution stage ...

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Abstract

The invention relates to the technical field of biomedical science, and provides spider silk protein bone nails and a preparation method thereof. The preparation method of the spider silk protein bonenails comprises the following steps of (1) preparing a spider silk protein solution; and (2) injecting the spider silk protein solution into a mold, performing treatment with methanol or ethanol, andperforming drying so as to obtain the spider silk protein bone nails. The preparation method of the spider silk protein bone nails provided by the invention is simple to operate and can be in industrialized production; and the prepared spider silk protein bone nails have the advantages of being high in strength, high in tenacity, light, resistant to ultraviolet rays, biodegradable and the like, can be degraded into amino acids and polypeptide in human bodies, and do not have any toxic or harmful effects on the human bodies.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a spidroin protein bone nail and a preparation method thereof. Background technique [0002] Bone nails are bone fixation devices commonly used in bone repair procedures. Traditional bone nails are made of metal materials such as stainless steel, titanium and their alloy materials. Although these materials have the advantages of high strength and good toughness, after the patient recovers, a second operation is required to take out the metal bone nails, which brings pain to the patient. It is painful for the second time, and the cavity left after the second operation will also leave the patient with a potential risk of fracture. [0003] At present, degradable materials such as polylactic acid (PLA) and polyglycolide-lactide (PLGA) have been used in clinical bone repair. Although the bone nails prepared by absorbable polymer materials can overcome many defects of traditiona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/14A61L31/16C12P21/00C07K1/16C07K1/14
Inventor 陶虎柳克银
Owner 江西丝科生物科技有限公司
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