A strain of Paenibacillus jemira, its fermentation product, preparation method and application

A technology of Bacillus and fermentation products, applied in the field of microorganisms, can solve the problems of affecting the quality of citrus, withering of branches, and reducing production of citrus, achieving good application prospects, inhibiting growth and colonization, and good stability

Active Publication Date: 2020-12-11
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lesions on the fruit will seriously affect the quality of citrus. When the disease is severe, it will lead to fruit drop, leaf fall, and branch withering, resulting in a reduction in citrus production.

Method used

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  • A strain of Paenibacillus jemira, its fermentation product, preparation method and application
  • A strain of Paenibacillus jemira, its fermentation product, preparation method and application
  • A strain of Paenibacillus jemira, its fermentation product, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Screening of X. citri antagonistic strain WAC199

[0042] 1. Preparation of LB solid medium containing Xac jx-6

[0043] Xanthomonas axonopodis pv.citrijx-6 strain, referred to as Xac jx-6, (the full-length sequence of the strain has been published in NCBI, the sequence number is CP011827.2), provided by the Laboratory of Population Microbiology Center, College of Agriculture, South China Agricultural University .

[0044] The activation medium of Xac jx-6 of citrus canker sores Xac jx-6 is LB solid medium, and a single colony was picked and inoculated in LB liquid medium, and cultured with shaking at 30°C.

[0045]Mix LB solid medium and Xac jx-6 bacterial suspension (OD600 about 1) at a temperature of 40-50°C at a ratio of 500 μL of bacterial suspension per 50 mL of LB solid medium, pour about 12 mL into each dish The medium after mixing is LB solid medium containing Xac jx-6.

[0046] 2. Screening of antagonistic strains against citrus canker

[0047] (...

Embodiment 2

[0050] Example 2 Identification of X. citri antagonistic strain WAC199

[0051] 1. Observation of the morphological characteristics of the strain

[0052] The preserved antagonistic strain WAC199 was inoculated on LB plates, cultured at 30°C for 24h-36h, and the characteristics of the colonies were observed. WAC199 grows well in LB medium, the colonies are off-white, raised, opaque, with irregular edges, Gram staining is positive, spores are middle or apical, spores are oval, and the cells are arranged singly or in pairs. The growth morphology and the morphology observed by microscope after Gram staining are as follows: figure 2 , image 3 shown.

[0053] 2.16S rDNA sequence analysis

[0054] Inoculate the preserved antagonistic strain WAC199 on an LB plate, culture at 30°C for 24h to 36h, and use the method of direct PCR amplification of a single bacterial colony, using 16S rDNA universal primers F27: 5'-AGAGTTTGATCATGGCTCAG-3', R1492: 5'- TACGGTTACCTTGTTACGACTT-3' was ...

Embodiment 3

[0062] Example 3 Media Screening for Optimal Production of Metabolic Active Substances of X. citri Antagonistic Strain WAC199

[0063] Prepare the following liquid media respectively:

[0064] (1) NYD liquid medium: beef extract 8g, yeast extract 3g, glucose 1g, distilled water 1000mL;

[0065] (2) YPG liquid medium: yeast extract 10g, peptone 20g, glucose 20g, distilled water 1 000mL;

[0066] (3) YPD liquid medium: yeast extract 10g, tryptone 20g, glucose 20g, distilled water 1 000mL;

[0067] (4) LB liquid medium: yeast extract 5g, peptone 20g, NaCl 20g, distilled water 1 000mL,

[0068] (5) LB broth liquid medium: LB broth medium (Haibo Biotechnology Co., Ltd., product number: HB0128) 30g, distilled water 1000mL;

[0069] (6) Corn flour liquid medium: 30 g of corn powder (Haibo Biotechnology Co., Ltd., product number: HBYL004), 1 000 mL of distilled water;

[0070] (7) Tryptone soy liquid medium: Tryptone soy liquid medium, Haibo Biotechnology Co., Ltd., product number...

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Abstract

The invention provides a strain of Paenibacillus jamilae, and a fermentation product, a preparation method and application thereof. The name of the strain of Paenibacillus jamilae is Paenibacillus jamilae WAC199 which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) located in Guangzhou of China on October 31, 2018, the preservation number is GDMCC No: 60470, and the strain ofPaenibacillus jamilae has a remarkable inhibition effect on citrus canker pathogens and sporisorium scitamineum. The invention also provides a fermentation culture method of a metabolic active substance of the strain, and the preparation method is simple and easy to operate; the metabolic active substance also has a remarkable antagonistic effect, and keeps high and stable bacteriostatic activityunder high temperature, a wide pH range and ultraviolet irradiation. The invention provides a more economic, effective and environment-friendly solution for biological prevention and control of citrus canker and sugarcane smut, and a good application prospect in prevention and control of pathogenic bacteria is provided.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of Paenibacillus jemira, a fermentation product thereof, a preparation method and an application thereof. Background technique [0002] Citrus canker is a plant quarantine disease at home and abroad. It is a bacterial plant disease caused by Xanthomonas axonopodispv.citri (Xac) pathogenic species of Xanthomonas axonopodispv. One of the main diseases of production. Citrus canker mainly damages the branches, leaves, and fruits of citrus. The most direct disease symptoms are yellow halos, raised and crater-shaped circular lesions on both sides. Lesions on the fruit will seriously affect the quality of citrus. When the disease is serious, it will lead to fruit drop, leaf fall, and branch withering, which will lead to a reduction in citrus production. In recent years, the prevention and control of citrus canker mainly relies on chemical pesticides. Extensive use of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/25A01P1/00A01P3/00C12R1/01
Inventor 王俊霞陈小云叶晓欣万高旭周佳暖常长青徐领会
Owner SOUTH CHINA AGRI UNIV
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